The VAMP-associated protein VAP27-1 plays a crucial role in plant resistance to ER stress by modulating ER–PM contact architecture in Arabidopsis  

作　　者：Yi Man Yue Zhang Linghui Chen Junhui Zhou Yufen Bu Xi Zhang Xiaojuan Li Yun Li Yanping Jing Jinxing Lin 

机构地区：[1]National Engineering Research Center of Tree Breeding and Ecological Restoration,College of Biological Sciences and Technology,Beijing Forestry University,Beijing 100083,China [2]Key Laboratory of Genetics and Breeding in Fore [3]These authors contributed equally to this article

出　　处：《Plant Communications》2024年第7期261-279,共19页植物通讯（英文）

基　　金：supported by the National Natural Science Foundation of China(32170689,91954202,32030010);National Key Research and Development Program of China(2022YFF0712500);the Program of Introducing Talents of Discipline to Universities(111 Project,B13007);Beijing Forestry University Outstanding Postgraduate Mentoring Team Building(YJSY-DSTD2022005).

摘　　要：The endoplasmic reticulum(ER)and the plasma membrane(PM)form ER–PM contact sites(EPCSs)that allow the ER and PM to exchange materials and information.Stress-induced disruption of protein folding triggers ER stress,and the cell initiates the unfolded protein response(UPR)to resist the stress.However,whether EPCSs play a role in ER stress in plants remains unclear.VESICLE-ASSOCIATED MEMBRANE PROTEIN(VAMP)-ASSOCIATED PROTEIN 27-1(VAP27-1)functions in EPCS tethering and is encoded by a family of 10 genes(VAP27-1–10)in Arabidopsis thaliana.Here,we used CRISPR-Cas9-mediated genome editing to obtain a homozygous vap27-1 vap27-3 vap27-4(vap27-1/3/4)triple mutant lacking three of the key VAP27 family members in Arabidopsis.The vap27-1/3/4 mutant exhibits defects in ER–PM connectivity and EPCS architecture,as well as excessive UPR signaling.We further showed that relocation of VAP27-1 to the PM mediates specific VAP27-1-related EPCS remodeling and expansion under ER stress.Moreover,the spatiotemporal dynamics of VAP27-1 at the PM increase ER–PM connectivity and enhance Arabidopsis resistance to ER stress.In addition,we revealed an important role for intracellular calcium homeostasis in the regulation of UPR signaling.Taken together,these results broaden our understanding of the molecular and cellular mechanisms of ER stress and UPR signaling in plants,providing additional clues for improving plant broad-spectrum resistance to different stresses.

关 键 词：ARABIDOPSIS VAP27-1 ER–PM contact sites ER stress unfolded protein response 

分 类 号：Q94[生物学—植物学]