Hydrogen peroxide sulfenylates and inhibits the photorespiratory enzyme PGLP1 to modulate plant thermotolerance  

作　　者：Zheng-Wei Fu Feng Ding Bing-Lei Zhang Wen-Cheng Liu Zi-Hong Huang Shi-Hang Fan Yu-Rui Feng Ying-Tang Lu Wei Hua 

机构地区：[1]Key Laboratory of Biology and Genetic Improvement of Oil Crops,Ministry of Agriculture and Rural Affairs,Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences,Wuhan 430062,China [2]State Key Laboratory of Hybrid Rice,College of Life Sciences,Wuhan University,Wuhan 430072,China [3]School of Landscape and Ecological Engineering,Hebei University of Engineering,Handan 056038,China [4]State Key Laboratory of Crop Stress Adaptation and Improvement,School of Life Sciences,Henan University,Kaifeng 475004,China [5]Hubei Hongshan Laboratory,Wuhan 430070,China

出　　处：《Plant Communications》2024年第6期139-153,共15页植物通讯（英文）

基　　金：supported by grants from the National Key Research and Development Program of China(2022YFD1200400);the National Natural Science Foundation of China(32301864 and 32322010).

摘　　要：Climate change is resulting in more frequent and rapidly changing temperatures at both extremes that severely affect the growth and production of plants,particularly crops.Oxidative stress caused by high temperatures is one of the most damaging factors for plants.However,the role of hydrogen peroxide(H_(2)O_(2))in modulating plant thermotolerance is largely unknown,and the regulation of photorespiration essential for C3 species remains to be fully clarified.Here,we report that heat stress promotes H2O2 accumulation in chloroplasts and that H2O2 stimulates sulfenylation of the chloroplast-localized photorespiratory enzyme 2-phosphoglycolate phosphatase 1(PGLP1)at cysteine 86,inhibiting its activity and promoting the accumulation of the toxic metabolite 2-phosphoglycolate.We also demonstrate that PGLP1 has a positive function in plant thermotolerance,as PGLP1 antisense lines have greater heat sensitivity and PGLP1-overexpressing plants have higher heat-stress tolerance than the wild type.Together,our results demonstrate that heat-induced H2O2 in chloroplasts sulfenylates and inhibits PGLP1 to modulate plant thermotolerance.Furthermore,targeting CATALASE2 to chloroplasts can largely prevent the heatinduced overaccumulation of H2O2 and the sulfenylation of PGLP1,thus conferring thermotolerance without a plant growth penalty.These findings reveal that heat-induced H2O2 in chloroplasts is important for heat-caused plant damage.

关 键 词：heat stress PHOTORESPIRATION hydrogen peroxide PGLP1 2-phosphoglycolate 

分 类 号：Q94[生物学—植物学]