Electrical stress and acid orange 7 synergistically clear the blockage of electron flow in the methanogenesis of low-strength wastewater  

作　　者：Ze-Chong Guo Min-Hua Cui Chun-Xue Yang Hong-Liang Dai Tong-Yi Yang Lin-Zhi Zhai Yong Chen Wen-Zong Liu Ai-Jie Wang 

机构地区：[1]School of Environmental and Chemical Engineering,Jiangsu University of Science and Technology,Zhenjiang,212100,China [2]State Key Laboratory of Urban Water Resource and Environment,School of Environment,Harbin Institute of Technology,Harbin,150090,China [3]Jiangsu Key Laboratory of Anaerobic Biotechnology,Jiangnan University,Wuxi,214122,China [4]School of Civil and Environmental Engineering,Harbin Institute of Technology,Shenzhen,518055,China [5]School of Geography and Tourism,Harbin University,Harbin,150001,China [6]School of Environmental Science and Engineering,Huazhong University of Science and Technology,Wuhan,430074,China

出　　处：《Environmental Science and Ecotechnology》2024年第4期291-299,共9页环境科学与生态技术（英文）

基　　金：supported by the National Natural Science Foundation of China(No.52000090 and No.52370171);the National Science Foundation of China(No.52321005);the China Postdoctoral Science Foundation(No.2021M701511);the Shenzhen Overseas High-level Talents Research Startup Program from Harbin Institute of Technology(Shenzhen);the Natural Science Foundation of Guangdong Province for Distinguished Young Scientists(No.2021B1515020084).

摘　　要：Energy recovery from low-strength wastewater through anaerobic methanogenesis is constrained by limited substrate availability.The development of efficient methanogenic communities is critical but challenging.Here we develop a strategy to acclimate methanogenic communities using conductive carrier(CC),electrical stress(ES),and Acid Orange 7(AO7)in a modified biofilter.The synergistic integration of CC,ES,and AO7 precipitated a remarkable 72-fold surge in methane production rate compared to the baseline.This increase was attributed to an altered methanogenic community function,independent of the continuous presence of AO7 and ES.AO7 acted as an external electron acceptor,accelerating acetogenesis from fermentation intermediates,restructuring the bacterial community,and enriching electroactive bacteria(EAB).Meanwhile,CC and ES orchestrated the assembly of the archaeal community and promoted electrotrophic methanogens,enhancing acetotrophic methanogenesis electron flow via a mechanism distinct from direct electrochemical interactions.The collective application of CC,ES,and AO7 effectively mitigated electron flow impediments in low-strength wastewater methanogenesis,achieving an additional 34%electron recovery from the substrate.This study proposes a new method of amending anaerobic digestion systems with conductive materials to advance wastewater treatment,sustainability,and energy self-sufficiency.

关 键 词：METHANOGENESIS Conductive carrier Electrical stress Acid orange 7 Electron transfer 

分 类 号：X703[环境科学与工程—环境工程]