An In Vitro and In Vivo Study of Thyroid Disruption of 1,2-Bis(2,4,6-tribromophenoxy)ethane(BTBPE)�A Novel Brominated Flame Retardant  

作　　者：Na Zheng Na Li Lei Lei Biran Zhu Kun Qiao Qiangwei Wang Chengqian Liang Yongyong Guo Lihua Yang Jian Han Yuxi Zhou Bingsheng Zhou 

机构地区：[1]State Key Laboratory of Freshwater Ecology and Biotechnology,Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan 430072,P.R.China [2]University of Chinese Academy of Sciences,Beijing 100049,P.R.China [3]Key Laboratory of Drinking Water Science and Technology,Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences,Beijing 100085,P.R.China [4]Institute of Pesticide and Environmental Toxicology,Zhejiang University,Hangzhou 310058,P.R.China

出　　处：《Environment & Health》2024年第1期42-51,共10页环境与健康（英文）

基　　金：supported by the National Natural Science Foundation of China (Nos.42277278,22276213).

摘　　要：The novel brominated flame retardant,1,2-bis-(2,4,6-tribromophenoxy)ethane(BTBPE),is an emerging environ-mental pollutant with undetermined toxicity.We investigated how BTBPE causes thyroid endocrine disruption with integrated in silico,in vitro,and in vivo assays.In yeast two-hybrid and T-Screen assays,BTBPE interacted with zebrafish thyroid hormone receptors with binding energies weaker than the TR agonist-3,3′,5-Triiodo-L-thyronine(T3),and disrupted thyroid function as a thyroid receptor(TR)agonist.We examined the bioconcentra-tion,developmental toxicity,and thyroid endocrine disruption in zebrafish after a 14-day exposure to BTBPE(1,3,10μg/L).Thyroxine(T4)was lower in BTBPE-treated larvae,whereas corticotropin-releasing hormone(CRH)and thyroid-stimulating hormone(TSH)were higher.The gene transcription alterations along the hypothalamic-pituitary-thyroid(HPT)axis were observed.Furthermore,reduced locomotion suggested that BTBPE imparts developmental neurotoxicity at zebrafish early developmental stage.Establishing that BTBPE has thyroid endocrine-disrupting effects is an important step for understanding and managing BTBPE toxicity.

关 键 词：1 2-bis(4 6-tribromophenoxy)ethane THYROID endocrine disrupting developmental neurotoxicity yeast two-hybrid assay T-screen assay zebrafish larvae 

分 类 号：R581[医药卫生—内分泌]