Electricity generation by Pseudomonas putida B6-2 in microbial fuel cells using carboxylates and carbohydrate as substrates  

作　　者：Xiaoyan Qi Huangwei Cai Xiaolei Wang Ruijun Liu Ting Cai Sen Wang Xueying Liu Xia Wang 

机构地区：[1]State Key Laboratory of Microbial Technology,Shandong University,Qingdao 266237,China [2]Chemical Engineering Department,Columbia University,New York,NY 10027,United States [3]Powerchina Renewable Energy Co.,Ltd.,Beijing,100101,China

出　　处：《Engineering Microbiology》2024年第2期24-32,共9页工程微生物学（英文）

基　　金：supported by grants from National Natural Science Foundation of China (32070097 and 91951202);National Key Research and Development Program of China (2019YFA0904800).

摘　　要：Microbial fuel cells(MFCs)employing Pseudomonas putida B6-2(ATCC BAA-2545)as an exoelectrogen have been developed to harness energy from various conventional substrates,such as acetate,lactate,glucose,and fructose.Owing to its metabolic versatility,P.putida B6-2 demonstrates adaptable growth rates on diverse,cost-effective carbon sources within MFCs,exhibiting distinct energy production characteristics.Notably,the anode chamber’s pH rises with carboxylates’(acetate and lactate)consumption and decreases with carbohydrates’(glucose and fructose)utilization.The MFC utilizing fructose as a substrate achieved the highest power density at 411 mW m^(−2).Initial analysis revealed that P.putida B6-2 forms biofilms covered with nanowires,contributing to bioelectricity generation.These microbial nanowires are likely key players in direct extracellular electron transport through physical contact.This study established a robust foundation for producing valuable compounds and bioenergy from common substrates in bioelectrochemical systems(BESs)utilizing P.putida as an exoelectrogen.

关 键 词：Microbial fuel cell Pseudomonas putida B6-2 Multi-substrate biodegradation Power generation Extracellular electron transfer 

分 类 号：TM911.45[电气工程—电力电子与电力传动]