Biocontrol agents modulate phyllosphere microbiota interactions against pathogen Pseudomonas syringae  

作　　者：Zhaoyue Yang Tianbo Liu Jianqiang Fan Yiqiang Chen Shaolong Wu Jingjing Li Zhenghua Liu Zhendong Yang Liangzhi Li Suoni Liu Hongwu Yang Huaqun Yin Delong Meng Qianjun Tang 

机构地区：[1]College of Plant Protection,Hunan Agricultural University,Changsha,410128,Hunan,China [2]School of Minerals Processing and Bioengineering,Central South University,Changsha,410083,Hunan,China [3]Hunan Tobacco Research Institute,Changsha,410004,Hunan,China [4]Technology Center,Fujian Tobacco Industrial Co.,Ltd.,Xiamen,361000,Fujian,China [5]School of Architecture and Civil Engineering,Chengdu University,Chengdu,610106,Sichuan,China [6]Yongzhou Tobacco Corporation,Yongzhou,425000,Hunan,China

出　　处：《Environmental Science and Ecotechnology》2024年第5期309-317,共9页环境科学与生态技术（英文）

基　　金：supported by the Key Project of Science and Technology Project of China National Tobacco Corporation(110202101027)[LS-11];the Key Research Project of Hunan Province(2023NK2019);the Natural Science Foundation of Changsha(kq2202089);the International Scientific and Technological Cooperation Base of Hunan(2018WK4019).

摘　　要：The pathogen Pseudomonas syringae,responsible for a variety of diseases,poses a considerable threat to global crop yields.Emerging biocontrol strategies employ antagonistic microorganisms,utilizing phyllosphere microecology and systemic resistance to combat this disease.However,the interactions between phyllosphere microbial dynamics and the activation of the plant defense system remain poorly understood.Here we show significant alterations in phyllosphere microbiota structure and plant gene expression following the application of biocontrol agents.We reveal enhanced collaboration and integration of Sphingomonas and Methylobacterium within the microbial co-occurrence network.Notably,Sphingomonas inhibits P.syringae by disrupting pathogen chemotaxis and virulence.Additionally,both Sphingomonas and Methylobacterium activate plant defenses by upregulating pathogenesis-related gene expression through abscisic acid,ethylene,jasmonate acid,and salicylic acid signaling pathways.Our results highlighted that biocontrol agents promote plant health,from reconstructing beneficial microbial consortia to enhancing plant immunity.The findings enrich our comprehension of the synergistic interplays between phyllosphere microbiota and plant immunity,offering potential enhancements in biocontrol efficacy for crop protection.

关 键 词：Biological control METAGENOME TRANSCRIPTOME Community structure Pathogen resistance 

分 类 号：X172[环境科学与工程—环境科学]