OsNLP3 enhances grain weight and reduces grain chalkiness in rice  

作　　者：Liang-Qi Sun Yu Bai Jie Wu Shi-Jun Fan Si-Yan Chen Zheng-Yi Zhang Jin-Qiu Xia Shi-Mei Wang Yu-Ping Wang Peng Qin Shi-Gui Li Ping Xu Zhong Zhao Cheng-Bin Xiang Zi-Sheng Zhang 

机构地区：[1]Division of Life Sciences and Medicine,Division of Molecular&Cell Biophysics,Hefei National Science Center for Interdisciplinary Sciences at the Microscale,University of Science and Technology of China,The Innovation Academy of Seed Design,Chinese Academy of Sciences,Hefei,Anhui Province 230027,China [2]Experimental Center of Engineering and Materials Science,University of Science and Technology of China,Hefei 230027,China [3]Rice Research Institute,Anhui Academy of Agricultural Science,Hefei,China [4]State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China,Rice Research Institute,Sichuan Agricultural University,Chengdu,Sichuan,China [5]Shanghai Key Laboratory of Plant Functional Genomics and Resources,Shanghai Chenshan Botanical Garden,Shanghai Chenshan Plant Science Research Center,Chinese Academy of Sciences,Shanghai,China

出　　处：《Plant Communications》2024年第10期82-99,共18页植物通讯（英文）

基　　金：supported by grants from the National Natural Science Foundation of China(32321001 to Z.Z.and C.-B.X.and 32100208 to J.W.);the Strategic Priority Research Program of the Chinese Academy of Sciences(XDA24010303);the Anhui Provincial Natural Science Foundation(2108085QC103);the Fundamental Research Funds for the Central Universities(WK9100000023).

摘　　要：Grain weight,a key determinant of yield in rice(Oryza sativa L.),is governed primarily by genetic factors,whereas grain chalkiness,a detriment to grain quality,is intertwined with environmental factors such as mineral nutrients.Nitrogen(N)is recognized for its effect on grain chalkiness,but the underlying molecular mechanisms remain to be clarified.This study revealed the pivotal role of rice NODULE INCEPTION-LIKE PROTEIN 3(OsNLP3)in simultaneously regulating grain weight and grain chalkiness.Our investigation showed that loss of OsNLP3 leads to a reduction in both grain weight and dimension,in contrast to the enhancement observed with OsNLP3 overexpression.OsNLP3 directly suppresses the expression of OsCEP6.1 and OsNF-YA8,which were identified as negative regulators associated with grain weight.Consequently,two novel regulatory modules,OsNLP3-OsCEP6.1 and OsNLP3-OsNF-YA8,were identified as key players in grain weight regulation.Notably,the OsNLP3-OsNF-YA8 module not only increases grain weight but also mitigates grain chalkiness in response to N.This research clarifies the molecular mechanisms that orchestrate grain weight through the OsNLP3-OsCEP6.1 and OsNLP3-OsNF-YA8 modules,highlighting the pivotal role of the OsNLP3-OsNF-YA8 module in alleviating grain chalkiness.These findings reveal potential targets for simultaneous enhancement of rice yield and quality.

关 键 词：OsNLP3 OsCEP6.1 OsNF-YA8 grain weight grain chalkiness RICE Oryza sativa L. 

分 类 号：S511[农业科学—作物学]