LAZY4 acts additively with the starch-statolithdependent gravity-sensing pathway to regulate shoot gravitropism and tiller angle in rice  

作　　者：Wenguang Wang Linzhou Huang Yuqi Song Songtao Gui Jiajia Cao Han Zhang Mengchen Du Jiaze Chen Zihao Wang Jie Zhou Xiangbing Meng Dali Zeng Jiayang Li Yonghong Wang 

机构地区：[1]National Key Laboratory of Wheat Improvement,College of Life Sciences,Shandong Agricultural University,Tai’an 271018,China [2]Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,Beijing 100101,China [3]University of Chinese Academy of Sciences,Beijing 100039,China [4]Yazhouwan National Laboratory,Sanya 572024,China [5]College of Advanced Agricultural Sciences,Zhejiang A&F University,Hangzhou 311300,China

出　　处：《Plant Communications》2024年第10期154-165,共12页植物通讯（英文）

基　　金：supported by grants from the National Key Research and Development Program of China(2022YFF1002903 to Y.W.);the Strategic Priority Research Program“Molecular Mechanism of Plant Growth and Development”of CAS(XDB27010100 to Y.W.);the National Natural Science Foundation of China(32372075 to L.H.);the Top Talents Program“One Case One Discussion”(Yishiyiyi to Y.W.)from Shandong Province。

摘　　要：Rice tiller angle is a key agronomic trait that has significant effects on the establishment of a high-yield rice population.However,the molecular mechanism underlying the control of rice tiller angle remains to be clarified.Here,we characterized the novel tiller-angle gene LAZY4(LA4)in rice through map-based cloning.LA4 encodes a C3H2C3-type RING zinc-finger E3 ligase localized in the nucleus,and an in vitro ubiquitination assay revealed that the conserved RING finger domain is essential for its E3 ligase activity.We found that expression of LA4 can be induced by gravistimulation and that loss of LA4 function leads to defective shoot gravitropism caused by impaired asymmetric auxin redistribution upon gravistimulation.Genetic analysis demonstrated that LA4 acts in a distinct pathway from the starch biosynthesis regulators LA2 and LA3,which function in the starch-statolith-dependent pathway.Further genetic analysis showed that LA4 regulates shoot gravitropism and tiller angle by acting upstream of LA1 to mediate lateral auxin transport upon gravistimulation.Our studies reveal that LA4 regulates shoot gravitropism and tiller angle upstream of LA1 through a novel pathway independent of the LA2-LA3-mediated gravity-sensing mechanism,providing new insights into the rice tiller-angle regulatory network.

关 键 词：AUXIN shoot gravitropism tiller angle LAZY4 RICE 

分 类 号：S511[农业科学—作物学]