UDP-glucosyltransferase 71C4 controls the flux of phenylpropanoid metabolism to shape cotton seed development  

作　　者：Yiwen Cao Zegang Han Zhiyuan Zhang Lu He Chujun Huang Jinwen Chen Fan Dai Lisha Xuan Sunyi Yan Zhanfeng Si Yan Hu Tianzhen Zhang 

机构地区：[1]Zhejiang Provincial Key Laboratory of Crop Genetic Resources,the Advanced Seed Institute,Plant Precision Breeding Academy,College of Agriculture and Biotechnology,Zhejiang University,Hangzhou,China [2]Hainan Institute,Zhejiang University,Sanya,China

出　　处：《Plant Communications》2024年第8期157-171,共15页植物通讯（英文）

基　　金：supported by grants from the Fundamental Research Funds for the Central Universities(226-2022-00100);the NSFC(32130075);Xinjiang Production and Construction Corps(2023AA008);Research Startup Funding from Hainan Institute of Zhejiang University(0202-6602-A12201).

摘　　要：Seeds play a crucial role in plant reproduction,making it essential to identify genes that affect seed development.In this study,we focused on UDP-glucosyltransferase 71C4(UGT71C4)in cotton,a member of the glycosyltransferase family that shapes seed width and length,thereby influencing seed index and seed cotton yield.Overexpression of UGT71C4 results in seed enlargement owing to its glycosyltransferase activity on flavonoids,which redirects metabolic flux from lignin to flavonoid metabolism.This shift promotes cell proliferation in the ovule via accumulation of flavonoid glycosides,significantly enhancing seed cotton yield and increasing the seed index from 10.66 g to 11.91 g.By contrast,knockout of UGT71C4 leads to smaller seeds through activation of the lignin metabolism pathway and redirection of metabolic flux back to lignin synthesis.This redirection leads to increased ectopic lignin deposition in the ovule,inhibiting ovule growth and development,and alters yield components,increasing the lint percentage from 41.42%to 43.40%and reducing the seed index from 10.66 g to 8.60 g.Our research sheds new light on seed size development and reveals potential pathways for enhancing seed yield.

关 键 词：UDP-GLUCOSYLTRANSFERASE seed development phenylpropanoid metabolism gene editing 

分 类 号：Q94[生物学—植物学]