Whole-genome resequencing identifies candidate genes and allelic variation in the MdNADP-ME promoter that regulate fruit malate and fructose contents in apple  

作　　者：Weihong Fu Lin Zhao Wanjun Qiu Xu Xu Meng Ding Liming Lan Shenchun Qu Sanhong Wang 

机构地区：[1]College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China [2]Xuzhou Institute of Agricultural Sciences in Xuhuai Region of Jiangsu,Xuzhou 221131,China

出　　处：《Plant Communications》2024年第9期168-183,共16页植物通讯（英文）

基　　金：upported by the National Key Research and Development Program of China,“Physiological basis and regulation of fruit tree quality and high yield”(2019YFD1000103);a Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD).

摘　　要：Soluble sugar and organic acids are key determinants of fruit organoleptic quality and directly affect the commodity value and economic returns of fruit crops.We performed whole-genome sequencing of the apple varieties Gala and Xiahongrou,along with their F1 hybrids,to construct a high-density bin map.Our quantitative genetic analysis pinpointed 53 quantitative trait loci(QTLs)related to 11 sugar and acid traits.We identified a candidate gene,MdNADP-ME,responsible for malate degradation,in a stable QTL on linkage group 15.Sequence analysis revealed an A/C SNP in the promoter region(MEp-799)that influences binding of the MdMYB2 transcription factor,thereby affecting MdNADP-ME expression.In our study of various apple genotypes,this SNP has been demonstrated to be linked to malate and fructose levels.We also developed a dCAPS marker associated with fruit fructose content.These results substantiate the role of MdNADP-ME in maintaining the equilibrium between sugar and acid contents in apple fruits.

关 键 词：APPLE quantitative trait locus malate degradation sugar content PROMOTER 

分 类 号：Q943.2[生物学—植物学]