The PRR-EC complex and SWR1 chromatin remodeling complex function cooperatively to repress nighttime hypocotyl elongation by modulating PIF4 expression in Arabidopsis  

作　　者：Jin Hoon Won Jeonghyang Park Hong Gil Lee Sangrae Shim Hongwoo Lee Eunkyoo Oh Pil Joon Seo 

机构地区：[1]Department of Chemistry,Seoul National University,Seoul 08826,Republic of Korea [2]Department of Life Sciences,Korea University,Seoul 02841,Republic of Korea [3]Plant Genomics and Breeding Institute,Seoul National University,Seoul 08826,Republic of Korea [4]Department of Forest Resources,Kangwon National University,Chuncheon 24341,Republic of Korea

出　　处：《Plant Communications》2024年第9期184-199,共16页植物通讯（英文）

基　　金：supported by the Basic Science Research(NRF2022R1A2B5B02001266 to P.J.S.and NRF-2023R1A2C3002386 to E.O.);Basic Research Laboratory(NRF-2022R1A4A3024451)programs provided by the National Research Foundation of Korea.

摘　　要：The circadian clock entrained by environmental light-dark cycles enables plants to fine-tune diurnal growth and developmental responses.Here,we show that physical interactions among evening clock components,including PSEUDO-RESPONSE REGULATOR 5(PRR5),TIMING OF CAB EXPRESSION 1(TOC1),and the Evening Complex(EC)component EARLY FLOWERING 3(ELF3),define a diurnal repressive chromatin structure specifically at the PHYTOCHROME-INTERACTING FACTOR 4(PIF4)locus in Arabidopsis.These three clock components act interdependently as well as independently to repress nighttime hypocotyl elongation,as hypocotyl elongation rate dramatically increased specifically at nighttime in the prr5-1 toc1-21 elf3-1 mutant,concomitantly with a substantial increase in PIF4 expression.Transcriptional repression of PIF4 by ELF3,PRR5,and TOC1 is mediated by the SWI2/SNF2-RELATED(SWR1)chromatin remodeling complex,which incorporates histone H2A.Z at thePIF4 locus,facilitating robust epigenetic suppression ofPIF4 during the evening.Overall,these findings demonstrate that the PRR-EC-SWR1 complex represses hypocotyl elongation at night through a distinctive chromatin domain covering PIF4 chromatin.

关 键 词：hypocotyl elongation circadian clock PRR Evening Complex histone H2A.Z SWR1 complex 

分 类 号：Q94[生物学—植物学]