饲料中添加包膜丁酸钠对蛋雏鸡盲肠原核微生物菌群的影响研究  

The effect of adding coated sodium butyrate in feed on the prokaryotic microbiota of cecum of chicks

作　　者：刘天强 孙才云 王思芦[2] 陈彬龙 胡耀东 张谊[2] LIU Tianqiang;SUN Caiyun;WANG Silu;CHEN Binlong;HU Yaodong;ZHANG Yi(Tongwei Agricultural Development Co.,Ltd.,Chengdu 610064,China;College of Animal Science,Xichang University,Xichang 615013,China)

机构地区：[1]通威农业发展有限公司,四川成都610041 [2]西昌学院动物科学学院,四川西昌615013

出　　处：《畜禽业》2024年第10期1-8,共8页Livestock and Poultry Industry

基　　金：西昌学院博士科研启动项目(YBZ202332)。

摘　　要：目的探究在饲料中添加丁酸钠对蛋雏鸡盲肠原核微生物菌群多样性的影响。方法研究选取1日龄罗曼粉蛋鸡,随机分为对照组与试验组,对照组使用标准饲料,试验组饲料中添加1500 mg/kg 30%包膜丁酸钠,其余饲养条件相同。在12、18、24、30日龄时对雏鸡进行称重并记录生长数据;随机选取正常生长的1月龄雏鸡,断头法处死并立即收集盲肠内容物,提取微生物总DNA,利用PCR扩增细菌16S rRNA基因的V3-V4高可变区域并通过Illumina HiSeq测序平台进行高通量测序。结果添加包膜丁酸钠的试验组雏鸡在生长后期的体质量显著增加;α多样性分析表明2组样品间ACE、Chao、Simpson和Shannon指数均未表现出显著差异(p>0.05);在门分类水平上,拟杆菌门(Bacteroidetes)、厚壁菌门(Frimicutes)和变形菌门(Proteobacteria)在盲肠细菌菌群中表现为优势菌群,但在试验组中具有更多的拟杆菌门及更少的厚壁菌门;在属分类水平上,试验组中拟杆菌属(Bacteroides)和乳酸杆菌属(Lactobacillus)的含量显著增加(p<0.05),硫杆菌属(Desulfovibrio)和厌氧螺菌属(Anaerobiospirillum)的含量显著降低(p<0.05)。结论作为新型非抗生素促生长饲料添加剂,包膜丁酸钠并未显著影响雏鸡盲肠原核微生物菌群多样性,但是显著影响了微生物菌属的比例,可改变蛋雏鸡盲肠微生物的组成,有助于改善肠道健康和盲肠菌群。Objective To investigate the impact of adding coated sodium butyrate to the feed on the diversity of prokaryotic microbiota of cecum of chicks.Method This study selected one-day-old Roman Brown chicks randomly divided into a control group and an experimental group.The control group received a standard diet,while the experimental group’s feed contained 1500 mg/kg of 30%coated sodium butyrate,with all other rearing conditions being the same,and recorded the chick growth data of the two groups in 12 days,18 days,24 days and 30 days respectively.After one month,randomly selected healthy growing chicks were euthanized,and their cecal contents were immediately collected.Microbial total DNA was extracted,and the V3-V4 hypervariable region of the bacterial 16S rRNA gene was amplified using PCR and subjected to high-throughput sequencing on the Illumina HiSeq platform.Result The analysis results showed that the chicks in the experimental group,with the addition of coated sodium butyrate,exhibited a significant increase in body weight during the later stages of growth.αdiversity analysis indicated that there were no significant differences in ACE,Chao,Simpson,and Shannon indices between the two groups(p>0.05).At the phylum level,Bacteroidetes,Firmicutes,and Proteobacteria were the dominant phyla in the cecal bacterial community,with the experimental group having a higher proportion of Bacteroidetes and a lower proportion of Firmicutes.At the genus level,the experimental group showed a significant increase in the abundance of Bacteroides and Lactobacillus(p<0.05),while the abundance of Desulfovibrio and Anaerobiospirillum decreased significantly(p<0.05).Conclusion As a novel non-antibiotic growth-promoting feed additive,coated sodium butyrate did not significantly affect the diversity of prokaryotic microbiota of cecum of chicks.However,it did have a significant impact on the proportions of microbial genera,altering the composition of the cecum microbe.This may contribute to improving gut health and cecal microbial commu

关 键 词：包膜丁酸钠 蛋雏鸡 盲肠微生物 高通量测序 

分 类 号：S831.5[农业科学—畜牧学]