玉米无基因型限制遗传转化体系建立和应用  

Genotype-independent transformation technique development and application in maize

作　　者：杨雅文 朱东杰 潘弘 张云涛 夏梦吟 韩宝柱 金敏亮 李梦娇 董鲁朋 杨宁[1,2,6] 周英 许洁婷 严建兵 YANG Ya-Wen;ZHU Dong-Jie;PAN Hong;ZHANG Yun-Tao;XIA Meng-Yin;HAN Bao-Zhu;JIN Min-Liang;LI Meng-Jiao;DONG Lu-Peng;YANG Ning;ZHOU Ying;XU Jie-Ting;YAN Jian-Bing(National Key Laboratory of Crop Genetic Improvement/Huazhong Agricultural University,Wuhan 430070,Hubei,China;Hubei Hongshan Labo-ratory,Wuhan 430070,Hubei,China;XINMI Biotechnology Co.,Ltd.,Changzhou 213000,Jiangsu,China;Yazhouwan National Laboratory,Sanya 572024,Hainan,China;Institute of Agricultural Sciences of Xishuangbanna Prefecture of Yunnan Province,Jinghong 666100,Yunnan,China;Yan Jianbing Expert Workstation of Yunnan Province,Jinghong 666100,Yunnan,China)

机构地区：[1]华中农业大学/作物遗传改良全国重点实验室,湖北武汉430070 [2]湖北洪山实验室,湖北武汉430070 [3]新米生物科技有限公司,江苏常州213000 [4]崖州湾国家实验室,海南三亚572000 [5]西双版纳傣族自治州农业科学研究所,云南景洪666100 [6]云南省严建兵专家工作站,云南景洪666100

出　　处：《作物学报》2024年第11期2674-2683,共10页Acta Agronomica Sinica

基　　金：国家自然科学基金项目(32321005);云南省严建兵专家工作站(202305AF150111);江苏省农业科技自主创新资金项目(CX(21)1003)资助。

摘　　要：农杆菌介导的玉米自交系遗传转化具有基因型依赖性。形态发生基因Baby boom(Bbm)和Wuschel2(Wus2)显著提高了转化效率,拓宽了可转化自交系的范围。然而,多数玉米自交系仍然难以得到转基因苗,且潜在机制尚不清楚。本研究发现,目标载体与Bbm和Wus2的辅助载体按10∶1比例混合能使大部分自交系产生体细胞胚。瞬时侵染效率和筛选是影响体细胞胚形成和成苗的关键因素。通过利用Bbm和Wus2混转以及优化侵染和延迟筛选的方式,建立了一个快速、不受基因型限制的玉米遗传转化体系。利用该技术体系对131个自交系进行遗传转化,其中104个自交系获得阳性转基因植株。The genetic transformation of maize inbred lines via Agrobacterium tumefaciens is highly genotype-dependent.The morphogenetic genes Baby boom(Bbm)and Wuschel2(Wus2)significantly enhance transformation efficiency and expand the range of amenable inbred lines.However,achieving transgenic seedlings remain challenging for many maize inbred lines,and the underly-ing mechanism remains unclear.In this study,we found that mixing the target vector with Bbm and Wus2 in a 10:1 ratio facilitates the generation of somatic embryos in most inbred lines.Transient transfection efficiency and the timing of selection are critical factors influencing the formation of somatic embryos and subsequent seedling development.By optimizing infection conditions and delaying selection,we established an efficient and rapid genetic transformation system that is not restricted by genotype.Using this system,we conducted genetic transformation on 131 inbred lines,resulting in successful transgenic plants in 104 of these lines.

关 键 词：玉米 遗传转化 形态发生基因 侵染效率 筛选 

分 类 号：S51[农业科学—作物学]