MeTCP3a转录因子在木薯叶片发育中的功能鉴定  

Functional identification of MeTCP3a transcription factor in cassava leaf development

作　　者：王连南 李远超 余乃通[2] 麦伟涛 李亚军[2] 陈新[2] WANG Lian-Nan;LI Yuan-Chao;YU Nai-Tong;MAI Wei-Tao;LI Ya-Jun;CHEN Xin(College of Tropical Agriculture and Forestry,Hainan University,Haikou 570228,Hainan,China;Institute of Tropical Bioscience and Biotech-nology,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101,Hainan,China;College of Life Science and Technology,Guangxi University,Nanning 530004,Guangxi,China)

机构地区：[1]海南大学热带农林学院,海南海口570228 [2]中国热带农业科学院热带生物技术研究所,海南海口571101 [3]广西大学生命科学与技术与学院,广西南宁530004

出　　处：《作物学报》2024年第11期2720-2730,共11页Acta Agronomica Sinica

基　　金：中国热带农业科学院国家热带农业科学中心科技创新团队(CATASCXTD202301);财政部和农业农村部国家现代农业产业技术体系建设专项(木薯,CARS11-HNCX)资助。

摘　　要：叶片是绿色植物进行光合作用的主要器官,木薯叶片的发育间接影响其块根产量以及淀粉含量,但木薯叶片发育的调控机制并不明晰,本文探究了转录因子MeTCP3a在木薯叶片发育中的功能。氨基酸序列比对及进化树分析表明,MeTCP3a与橡胶(Hevea brasiliensis Muell.Arg)TCP4蛋白相似性最高。通过亚细胞定位和蛋白互作试验证实,MeTCP3a定位于细胞核,且具有转录激活能力,还能形成同源及异源二聚体,表明其具备转录因子特性。此外,不同组织部位表达分析发现,MeTCP3a在成熟叶片中表达活性最高,而通过VIGS技术在木薯叶片中将其沉默后,发现木薯心叶形态出现卷曲、皱缩。进一步实时荧光定量PCR分析发现,与叶片形态发育相关的下游基因MeCUC1/2/3和MeIAA的表达量也随之下调,表明MeTCP3a可能通过调控下游基因的表达来影响叶片形态发育。本文为进一步探索TCP类转录因子在木薯叶片生长发育中的调控机制提供了理论依据。Leaves are the primary organs of photosynthesis in green plants,and their development indirectly affects the yield and starch content of cassava storage roots.However,the regulatory mechanisms governing cassava leaf development remain unclear.In this study,we explored the function of the MeTCP3a transcription factor in cassava leaf development.Amino acid sequence alignment and phylogenetic analysis showed that MeTCP3a was closely related to the TCP4 protein in rubber(Hevea brasiliensis Muell.Arg.).Subcellular localization and protein interaction assays demonstrated that MeTCP3a was localized in the nucleus,possessed transcriptional activation ability,and could form both homodimers and heterodimers in the nucleus,indicating its properties as a transcription factor.Expression analysis showed that the relative expression level of MeTCP3a was significantly higher in mature leaves compared to other tissues.When MeTCP3a expression was suppressed using VIGS(virus-induced gene silencing)technology,cassava heart leaves exhibited curled edges and a wrinkled appearance.Further real-time quantitative PCR analysis revealed that the expression levels of downstream genes related to leaf morphological development,such as MeCUC1/2/3 and MeIAA,were also down-regulated.This result suggested that MeTCP3a might influ-ence leaf morphological development by regulating the expression of these downstream genes.This study provides a theoretical basis for further exploring the regulatory mechanisms of TCP-like transcription factors associated with leaf growth and devel-opment in cassava.

关 键 词：木薯 叶片发育 MeTCP3a 蛋白互作 VIGS 

分 类 号：S53[农业科学—作物学]