抑制交替氧化酶对水稻稻瘟菌防治的影响初探  

A preliminary study on the effect of alternative oxidase(AOX)inhibition on the control of Magnaporthe oryzae

作　　者：徐飞[1] 刘杨 许健成 余璐璐[1] XU Fei;LIU Yang;XU Jian-Cheng;YU Lu-Lu(Wuhan University of Bioengineering/Applied Biotechnology Center,Wuhan 430415,Hubei,China)

机构地区：[1]武汉生物工程学院/应用生物技术研究中心,湖北武汉430415

出　　处：《作物学报》2024年第11期2908-2916,共9页Acta Agronomica Sinica

基　　金：国家自然科学基金项目(31900242);湖北省自然科学基金面上项目(2022CFB009)资助。

摘　　要：水稻(Oryza sativa L.)是世界主要粮食作物之一。中国作为世界上最大的稻米生产国,每年因稻瘟菌(Magnaporthe oryzae)引起的稻瘟病受到广泛关注。稻瘟病在水稻整个生育期中均可发生,从而引起水稻大幅度减产,甚至颗粒无收。值得注意的是,交替氧化酶(alternative oxidase,AOX)广泛存在于植物和真菌中,是线粒体呼吸电子传递链中的末端氧化酶,被证实参与了胁迫条件下植物的生长发育调节以及真菌胁迫响应等。本文比较分析了水稻AOX和稻瘟菌AOX蛋白结构差异,并利用AOX抑制剂开展稻瘟菌防治试验。结果表明,水稻AOX和稻瘟菌AOX蛋白的双铁催化活性中心的氨基酸残基相对保守,但其在泛醌通道大小、氨基酸组成以及形成的疏水口袋等方面有较大差异。通过AOX抑制剂处理可明显减缓稻瘟菌菌丝的生长,并减轻水稻叶片稻瘟菌病害症状。本研究表明,AOX可作为有效靶标用于抑制稻瘟菌,在水稻稻瘟菌病害防治方面具有潜在应用价值。Rice(Oryza sativa L.)is one of the world’s major food crops.As the largest rice producer globally,China faces sig-nificant challenges annually due to rice blast caused by Magnaporthe oryzae.This disease can affect rice throughout its entire growth period,leading to substantial yield reductions and even total crop failure.Notably,alternative oxidase(AOX),a terminal oxidase in the mitochondrial respiratory electron transport chain,is widely present in both plants and fungi.AOX is known to play a role in regulating plant growth and development under stress conditions and in the fungal stress response.In this study,we conducted a comparative analysis of the structural differences between rice AOX and M.oryzae AOX proteins and employed AOX inhibitors to assess their potential in inhibiting and controlling M.oryzae.Our results revealed that while the amino acid residues in the dual-iron catalytic active center of rice and M.oryzae AOX proteins are relatively conserved,there are significant differ-ences in the size of the ubiquinone channel,amino acid composition,and the shape of the hydrophobic pocket.Treatment with AOX inhibitors significantly slowed the growth of M.oryzae hyphae and reduced the severity of disease symptoms on rice leaves.These findings suggest that AOX is a promising target for inhibiting M.oryzae,offering potential application value in the control of rice blast disease.

关 键 词：水稻 交替氧化酶 稻瘟菌 病害防治 抑制剂 

分 类 号：S51[农业科学—作物学]