Investigating the Genetic Bases of Growth Regulation by E2F3 in Dwarf Surf Clams Mulinia lateralis  

作　　者：YAO Ruixing WANG Chen KONG Lingling WANG Yujue BAO Zhenmin HU Xiaoli 

机构地区：[1]MOE Key Laboratory of Marine Genetics and Breeding,College of Marine Life Sciences,Ocean University of China,Qingdao 266003,China [2]Laboratory for Marine Fisheries Science and Food Production Processes,Qingdao Marine Science and Technology,Center,Qingdao 266237,China [3]Key Laboratory of Tropical Aquatic Germplasm of Hainan Province,Sanya Oceanographic Institution,Ocean University of China,Sanya 572000,China

出　　处：《Journal of Ocean University of China》2024年第5期1347-1358,共12页中国海洋大学学报（英文版）

基　　金：funded by the National Natural Science Foundation of China (No. U2106231);the Key Research and Development Project of Shandong Province (No. 2021 ZLGX03);the National Key Research and Development Program of China (No. 2022YFD2400303)

摘　　要：Bivalve aquaculture plays a crucial role in the aquaculture industry due to the economic value of many bivalve species.Understanding the underlying genetic basis of bivalve growth regulation is essential for enhancing germplasm innovation and ensuring sustainable development of the industry.Though numerous candidate genes have been identified,their functional validation remains challenging.Fortunately,the dwarf surf clam(Mulinia lateralis)serves as a promising model organism for investigating genetic mechanisms underlying growth regulation in bivalves.The GWAS study in the Yesso scallop(Patinopecten yessoensis)has pinpointed the E2F3 gene as a key regulator of growth-related traits.However,the specific role of E2F3 in bivalve growth remains unclear.This study aimed to further confirm the regulatory function of the E2F3 gene in the dwarf surf clam through RNA interference experiments.Our results revealed several genes are associated with individual growth and development,including CTS7,HSP70B2,and PGLYRP3,as well as genes involved in lipid metabolism such as FABP2 and FASN.Functional enrichment analysis indicated that E2F3 primarily modulates critical processes like amino acid and lipid metabolism.These findings suggest that E2F3 likely regulates growth in the dwarf surf clam by influencing amino acid and lipid metabolism.Overall,this study advances our understanding on the function of E2F3 gene in growth regulation in bivalves,providing valuable insights for future research in this field.

关 键 词：growth regulation E2F3 RNA interference experiment transcriptomic analysis dwarf surf clam Mulinia lateralis BIVALVE 

分 类 号：S917.4[农业科学—水产科学]