白刺花叶总生物碱通过抑制MAPK/NF-κB信号通路减轻脂多糖诱导的RAW264.7细胞炎症反应  

Sophora davidii Hance leaves total alkaloids Attenuate Lipopolysaccharide-induced inflammatory response in RAW264.7 cell by Inhibiting the MAPK/NF-κB signaling pathway

作　　者：姜盛楠 支文冰 陈静[2] 孙婷婷[1] 许宗仁 刘帅 张红[1] 李晔[1] 刘洋[1] JIANG Shengnan;ZHI Wenbing;CHEN Jing;SUN Tingting;XU Zongren;LIU Shuai;ZHANG Hong;LI Ye;LIU Yang(Shaanxi Academy of Traditional Chinese Medicine,Xi'an 710003,Shaanxi,China;不详)

机构地区：[1]陕西省中医药研究院(陕西省中医医院),陕西西安710003 [2]陕西中医药大学,陕西咸阳712046

出　　处：《实用医学杂志》2024年第20期2835-2840,共6页The Journal of Practical Medicine

基　　金：陕西省重点研发计划(编号:2021ZDLSF04-06、2022ZDXM-SF-06);陕西省自然科学基础研究计划(编号:2023-JC-QN-0820);陕西省中医药管理局科研项目(编号:SZY-KJCYC-2023-014);陕西省“秦药”研发重点实验室(编号:2021-QYPT-001);西安市科技计划项目(编号:23YXYJ0165);陕西中医药大学省级中医体质与疾病防治研究重点实验室(编号:KF2209)。

摘　　要：目的探讨白刺花叶总生物碱(Sophora davidii Hance leaves total alkaloids,SDLTAs)的体外抗炎作用及可能的分子机制。方法采用脂多糖(LPS)诱导RAW264.7细胞炎症模型,给予不同浓度(50、100、200μg/mL)SDLTAs,Griess法检测SDLTAs对细胞NO表达的影响;ELISA法检测SDLTAs对细胞因子IL-6、TNF-α和IL-1β表达的影响;RT-qPCR法检测细胞中iNOS、NF-κB p65和IκBαmRNA的表达;Western blotting法检测细胞中p-p38、p-p65和p-JNK以及细胞核内NF-κB p65的蛋白表达。结果SDLTAs显著抑制LPS诱导的RAW264.7细胞炎症反应。SDLTAs极显著降低细胞中NO、IL-6、TNF-α和IL-1β的分泌(P<0.01),极显著下调细胞中iNOS、NF-κB p65和IκBαmRNA的表达(P<0.01),极显著降低细胞中p-p38、p-p65和p-JNK以及细胞核内NF-κB p65的蛋白表达(P<0.01)。结论SDLTAs可通过调节MAPK/NF-κB信号通路发挥抗炎作用。Objective To investigate the in vitro anti-inflammatory effects of Sophora davidii Hance leaves total alkaloids(SDLTAs)and possible molecular mechanisms.Methods The lipopolysaccharide(LPS)-induced inflammation model of RAW264.7 cells was used,and different concentrations of SDLTAs(50,100 and 200μg/mL)were administered,and the effect of SDLTAs on cellular NO expression was detected by the Griess method;ELISA method was used to detect the effect of SDLTAs on the expression of IL-6,TNF-αand IL-1β;The expression of iNOS,NF-κB p65 and IκBαmRNA was detected by RT-qPCR;Western blotting was used to detecte the expression of p-p38,p-p65 and p-JNK in the cells and NF-κB p65 in the nucleus.Results SDLTAs could significantly inhibit the LPS-induced inflammatory response in RAW264.7 cells.SDLTAs significantly decreased the secretion of NO,IL-6,TNF-αand IL-1βin cells(P<0.01),and significantly decreased the mRNA expressions of iNOS,NF-κB p65 and IκBαin cells(P<0.01).Significantly decreased the protein expression of p-p38,p-p65 and p-JNK in cells and NF-κB p65 in nucleus(P<0.01).Conclusion SDLTAs can exert anti-inflammatory effects by regulating the MAPK/NF-κB signalling pathway.

关 键 词：白刺花叶 总生物碱 抗炎 作用机制 

分 类 号：R285[医药卫生—中药学]