男性不育患者精浆弹性蛋白酶水平与体外受精-胚胎移植结局的关系  

Relationship between seminal plasma elastase levels and in vitro fertilization-embryo transfer outcomes in male infertility patients

作　　者：谢立汉 胡丽琴 张昭 谢斯盛[1] XIE Lihan;HU Liqin;ZHANG Zhao;XIE Sisheng(Department of Reproductive Medicine,Dongguan People′s Hospital,Dongguan 523000,Guangdong,China;Department of Traditional Chinese Medicine,Dongguan People′s Hospital,Dongguan 523000,Guangdong,China)

机构地区：[1]东莞市人民医院生殖医学科,广东东莞523000 [2]东莞市人民医院中医科,广东东莞523000

出　　处：《中国性科学》2024年第10期34-37,共4页Chinese Journal of Human Sexuality

基　　金：广东省中医药局科研项目(20231362)。

摘　　要：目的分析男性不育患者精浆弹性蛋白酶(SPE)水平与体外受精-胚胎移植(IVF-ET)结局的关系。方法选取2020年6月至2022年12月东莞市人民医院收治的122例男性不育患者作为研究对象,根据IVF-ET治疗前患者SPE检测值分为异常组(n=40)和正常组(n=82)。比较两组常规精液参数和助孕结局,采用χ^(2)检验Phi系数分析SPE水平与IVF-ET结局的相关性,采用Pearson法分析SPE水平与精液白细胞浓度、精子DNA碎片率的相关性。结果异常组精液白细胞浓度、精子DNA碎片率、SPE水平均高于正常组,优质胚胎率、临床妊娠率均低于正常组(P<0.05)。男性不育患者SPE水平与优质胚胎率、临床妊娠率有关(P<0.05)。SPE水平与精液白细胞浓度、精子DNA碎片率呈正相关(P<0.05)。结论男性不育患者SPE水平与IVF-ET结局有关,且SPE异常高表达会降低优质胚胎率和临床妊娠率。Objective To analyze the relationship between seminal plasma elastase(SPE)levels and in vitro fertilization-embryo transfer(IVF-ET)outcomes in male infertility patients.Methods A total of 122 male infertility patients admitted to Dongguan People′s Hospital from June 2020 to December 2022 were selected as the research objects.According to the SPE detection values of patients before IVF-ET treatment,they were divided into abnormal group(n=40)and normal group(n=82).The conventional semen parameters and assisted pregnancy outcomes were compared between the two groups.The correlation between the SPE level and IVF-ET outcome was analyzed byχ^(2) test Phi coefficient.The correlation between the SPE level and the semen white blood cell concentration and sperm DNA fragmentation index was analyzed by Pearson method.Results The abnormal group had higher semen leukocyte concentration,sperm DNA fragmentation index,and SPE level than the normal group,while the high-quality embryo rate and clinical pregnancy rate were lower than those of the normal group(P<0.05).The SPE level in male infertility patients was related to the rate of high-quality embryos and clinical pregnancy rate(P<0.05).The SPE levels were positively correlated with semen leukocyte concentration and sperm DNA fragmentation index(P<0.05).Conclusions The level of SPE in male infertility patients is related to the outcomes of IVF-ET,and abnormal high expression of SPE can reduce the high-quality embryo rate and clinical pregnancy rate of patients.

关 键 词：不育 体外受精-胚胎移植 精浆弹性蛋白酶 优质胚胎率 临床妊娠率 

分 类 号：R698[医药卫生—泌尿科学]