MPO-AAV患者外周血中性粒细胞胞内MPO表达及其临床意义  

Expression of Myeloperoxidase in Peripheral Blood Neutrophils and Its Clinical Significance in Patients with MPO-AAV

作　　者：李萃萃 赵义学 徐雪勤 LI Cuicui;ZHAO Yixue;XU Xueqin(Medical Laboratory Department,Zhumadian Central Hospital,Zhumadian 463000,China)

机构地区：[1]驻马店市中心医院医学检验科,河南驻马店463000

出　　处：《河南医学研究》2024年第19期3529-3533,共5页Henan Medical Research

摘　　要：目的探讨髓过氧化物酶(MPO)抗中性粒细胞胞浆抗体(ANCA)相关性血管炎(AAV)患者外周血中性粒细胞胞内MPO表达及其临床意义。方法将选取2021年6月至2022年12月在驻马店市中心就诊的85例MPO-AAV患者纳入病例组,同时期收集在医院体检健康的志愿者30名纳入对照组。收集患者的基础资料,采用ELISA法检测血清MPO,采用流式细胞术检测中性粒细胞胞膜MPO和胞内MPO表达。统计两组外周血中性粒细胞MPO表达、血清MPO水平,Spearman相关性分析外周血中性粒细胞MPO表达与血清MPO水平的关系,多元回归分析伯明翰血管炎活动评分(BVAS-V3)影响因素。结果两组外周血中性粒细胞胞膜MPO表达差异无统计学意义(P>0.05),病例组外周血中性粒细胞胞内MPO表达低于对照组(P<0.05)。病例组血清MPO水平高于对照组血清MPO水平(P<0.05)。中性粒细胞胞内MPO表达与血清MPO水平呈负相关(r=-0.711,P<0.05)。多元回归分析显示,BVAS-V3与中性粒细胞胞内MPO表达、MPO-ANCA呈正相关(P<0.05),与年龄、病程、红细胞沉降率、C反应蛋白、中性粒细胞计数、中性粒细胞胞膜MPO表达、血清MPO表达均无相关性(P>0.05)。结论MPO-AAV患者外周血中性粒细胞胞内MPO表达上升,MPO-ANCA阳性可能干预了中性粒细胞胞内MPO表达,且中性粒细胞胞内MPO表达、MPO-ANCA均为影响BVAS-V3的独立因素。Objective To investigate the expression of myeloperoxidase(MPO)in peripheral blood neutrophils and its clinical significance in patients with MPO-anti-neutropil cytoplasmic antibodies(ANCA)associated vasculitis(AAV).Methods A total of 85 MPO-AAV patients treated in Zhumadian Central Hospital from June 2021 to December 2022 were selected to be included in the case group,and 30 healthy volunteers who were collected in hospital during the same period were included in the control group.The basic data of patients were collected,serum MPO was detected by ELISA,neutrophil membrane MPO and intracellular MPO expression were detected by flow cytometry.Peripheral blood neutrophil MPO expression and serum MPO level were analyzed in the two groups.Spearman correlation analysis was conducted to determine the relationship between peripheral blood neutrophil MPO expression and serum MPO level,and multiple regression analysis was performed to analyze the influencing factors of Birmingham vasculitis activity score-version 3(BVAS-V3).Results There was no statistical significance in MPO expression in peripheral blood neutrophils between the two groups(P>0.05).The MPO expression in peripheral blood neutrophils in the case group was lower than that in the control group(P<0.05).The serum MPO level of the case group was higher than that of the control group(P<0.05).There was negative correlation between MPO expression in neutrophils and serum MPO level(r=-0.711,P<0.05).Multiple regression analysis showed that BVAS-V3 was positively correlated with neutrophil MPO expression and MPO-ANCA expression(P<0.05),but was not significantly correlated with age,disease course,erythrocyte sedimentation rate,C-reactive protein,neutrophil count,neutrophil membrane MPO expression,and serum MPO expression(P>0.05).Conclusion In patients with MPO-AAV,intracellular MPO expression in peripheral blood of neutrophils is significantly increased,and positive MPO-ANCA may interfere with the expression of MPO in neutrophils,and MPO-ANCA expression in neutrophils

关 键 词：抗髓过氧化物酶抗体 髓过氧化物酶 血管炎 

分 类 号：R446.1[医药卫生—诊断学]