Optimization of Enzymatic Extraction Process of Polysaccharides from Pseudostellaria heterophylla Fibrous Roots by Response Surface Methodology and Its Pilot Application  

作　　者：Huiqing PAN Qi ZHAO Yanda ZHANG Rongping YANG 

机构地区：[1]Fujian Provincial Enterprise Technology Center of Fujian Beidi Pharmaceutical Co.,Lid.,Ningde 355300,China [2]Fujian Beidi Pharmaceutical Co.,Ltd.,Ningde 355300,China [3]Zherong County TaiZiShen Association,Ningde 355300,China

出　　处：《Medicinal Plant》2024年第5期17-21,共5页药用植物（英文）

基　　金：Supported by Special Project of Central Leading Local Science and Technology Development(202113030);Regional Development Project of Fujian Provincial Science and Technology Plan(2022N3017).

摘　　要：[Objectives]To study and optimize the process conditions of enzymatic hydrolysis technology for extracting polysaccharides from Pseudostellaria heterophylla fibrous roots and its application in workshop pilot tests.[Methods]P.heterophylla fibrous roots were taken as the matrix material,and Box Behnken design was used to analyze the extraction time,composite enzyme addition amount,and liquid-solid ratio for response surface optimization experiments,and then applied to the pilot extraction of P.heterophylla fibrous roots.[Results]Response surface analysis showed that all factors had a significant impact on the experimental indicators.The optimal extraction process conditions for polysaccharides from P.heterophylla fibrous roots were extraction time of 2.7 h,compound enzyme addition of 2.5%,and liquid-solid ratio of 32.The yield of polysaccharides from P.heterophylla fibrous roots was 4.83%.The water extraction process of P.heterophylla fibrous roots extraction pilot was used as the control group for response surface optimization of the pilot experiment.The optimization results showed that the extraction time was 3 h,the amount of composite enzyme added was 2.5%,and the liquid-solid ratio was 28.The polysaccharide yield was 4.75%,an increase of 4.63%compared to the control group.[Conclusions]This paper could provide feasibility for the innovation of enzy-matic hydrolysis technology for P.heterophylla fibrous roots and its workshop pilot practice application,as well as a reference for the industrial application of its medicinal resources.

关 键 词：Pseudostellaria heterophylla fibrous roots POLYSACCHARIDES Enzymatic extraction Pilot production Response surface opti timization 

分 类 号：R284.2[医药卫生—中药学]