木薯MeYippee1基因的克隆、表达及互作蛋白研究  

Cloning,Expression,and Interaction Protein Study of MeYippee1 Gene in Cassava

作　　者：任治欣 陆小花 郑婉茹 梁宝娟 张慧敏 葛玉建 陈新[2,3] 姚远 耿梦婷[1] 郭建春 REN Zhixin;LU Xiaohua;ZHENG Wanru;LIANG Baojuan;ZHANG Huimin;GE Yujian;CHEN Xin;YAO Yuan;GENG Mengting;GUO Jianchun(Tropical Agriculture and Forestry College,Hainan University,Haikou,Hainan 570228,China;Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences/Hainan Institute for Tropical Agricultural Resources,Haikou,Hainan 571101,China;Sanya Research Institute,Chinese Academy of Tropical Agricultural Sciences,Sanya,Hainan 572000,China)

机构地区：[1]海南大学热带农林学院,海南海口570228 [2]中国热带农业科学院热带生物技术研究所/海南热带农业资源研究院,海南海口571101 [3]中国热带农业科学院三亚研究院,海南三亚572000

出　　处：《热带作物学报》2024年第10期2059-2066,共8页Chinese Journal of Tropical Crops

基　　金：国家自然科学基金项目(No.31960058);国家木薯产业技术体系项目(No.CARS11-HNGJC)。

摘　　要：解析木薯淀粉合成相关酶的表达调控机制,有助于高产、高淀粉木薯分子育种。前期研究发现,生长素快速响应基因MeSAUR1正调控淀粉合成关键酶AGPase的小亚基编码基因MeAGPS1a的表达;酵母双杂交筛选发现1个Yippee蛋白成员MeYippee1是MeSAUR1的候选互作蛋白。本研究克隆SC8木薯品种MeYippee1基因的编码区,全长321 bp,无内含子,编码106个氨基酸,编码的蛋白预测具有4个磷酸化位点。MeYippee1基因在不同组织器官中的表达分析发现,MeYippee1在腋芽中的表达量最高,其次为须根、块根和成熟叶,在茎和叶柄中的表达量较低,在顶芽和嫩叶中的表达量最低。MeYippee1在块根发育各时期的表达分析发现,MeYippee1主要在块根形成期表达。酵母双杂交点对点验证发现MeYippee1和MeSAUR1蛋白存在互作关系。本研究结果为MeSAUR1和MeYippee1互作正调控MeAGPS1a表达的分子机理解析提供新的线索。Understanding the expression regulation mechanisms of enzymes related to starch synthesis in cassava can significantly advance the molecular breeding for high yield and high starch content.Prior research has uncovered that the auxin-responsive gene,MeSAUR1,upregulates the expression of MeAGPS1a,a gene encoding the small subunit of the key starch-synthesizing enzyme,AGPase.Yeast two-hybrid screening identified a Yippee protein family member,MeYippee1,as a candidate interacting protein with MeSAUR1.In this study,the coding region of the MeYippee1 gene from cassava variety SC8 was cloned.The gene was 321 bp in the coding region,lacking introns,encoding a protein of 106 amino acids,with four predicted phosphorylation sites.Expression analysis in different tissues and organs revealed that MeYippee1was most highly expressed in axillary buds,followed by fibrous roots,storage roots,and mature leaves,while expression levels were lower in stems and petioles,and the lowest in apical buds and young leaves.Expression analysis during the different developmental stages of the storage root showed that MeYippee1 expression was primarily during the formation phase of the storage roots.Further point-to-point yeast two-hybrid verification indicated an interactive relationship between MeYippee1 and MeSAUR1 proteins.The results would provide new insights into the molecular mechanism underlying the positive regulation of MeAGPS1a expression by MeSAUR1.

关 键 词：MeYippee1 表达模式 酵母双杂交 

分 类 号：S533[农业科学—作物学]