黄瓜FtsZs基因家族鉴定及分析  

Identification and Analysis of FtsZs Family in Cucumber

作　　者：陈琳杰 常婧姝 张宇 颜爽 任仲海 王丽娜 CHEN Lin-jie;CHANG Jing-shu;ZHANG Yu;YAN Shuang;REN Zhong-hai;WANG Li-na(College of Horticultural Science and Engineering/Shandong Agricultural University's,along with the Shandong Fruit and Vegetable High-Quality and Efficient Production Collaborative Innovation Center and the Ministry of Agriculture's Key Laboratory of Horticultural Crop Biology and Germplasm Enhancement in the Huanghuai Region,are all located in Tai'an,Shandong Province,with the postal code 271018)

机构地区：[1]山东农业大学园艺科学与工程学院,山东果蔬优质高效生产协同创新中心,农业部黄淮地区园艺作物生物学与种质创制重点实验室,山东泰安271018

出　　处：《山东农业大学学报（自然科学版）》2024年第5期774-781,共8页Journal of Shandong Agricultural University：Natural Science Edition

基　　金：国家自然科学基金(31972419,31672170);山东“双一流”专项建设基金(SYL2017YSTD06);山东省泰山学者建设工程(ts20130932);山东省重点研发计划(农业良种工程)项目(2022LZGCQY001)。

摘　　要：FtsZ蛋白作为微管蛋白同源物,常通过自我组装成一个收缩的细胞骨架元素“Z”环,影响质体分裂。但是,黄瓜FtsZ蛋白的研究尚未见报道,其功能尚不明确。本文利用生物信息学方法在黄瓜中鉴定到了3个FtsZ蛋白,并将它们分为2组。序列分析发现它们的长度在4493~6264bp之间,含有6~7个外显子,氨基酸长度为421~488AA,分布在3号或6号染色体上;亚细胞定位预测结果显示,黄瓜FtsZ定位于叶绿体或细胞质;RNA-seq分析发现黄瓜FtsZs在茎、叶、雌花和子房表达较高,荧光定量PCR结果显示黄瓜CsFtsZ2-1基因在雄花中的表达量也较高,表明黄瓜FtsZs基因在叶、花和子房生长发育中发挥功能;此外,在胁迫响应过程中,CsFtsZ2-1基因可响应盐胁迫,CsFtsZ1、CsFtsZ2-1和CsFtsZ2-2可响应冷胁迫,CsFtsZ1和CsFtsZ2-1可响应白粉病病原菌侵染。本文章结果为进一步研究FtsZs基因在黄瓜中的作用和机制提供了参考和依据。FtsZ protein,as a homolog of tubulin,often assembles into a contractile cytoskeletal element known as the“Z”ring,influencing plastid division.However,research on cucumber FtsZ proteins has not been reported,and its function is unclear.In this paper,we identified three FtsZ proteins in cucumber by bioinformatics methods and classified them into two groups.Sequence analysis revealed that their lengths range from 4493 to 6264 bp,containing 6 to 7 exons,with amino acid lengths of 421 to 488 AA,located on chromosome 3 or 6;subcellular localization predictions indicated that cucumber FtsZ is located in the chloroplast or cytoplasm;RNA-seq analysis found that cucumber FtsZs are highly expressed in stem,leaf,female flower,and ovary,and fluorescence quantitative PCR results showed that the expression level of cucumber CsFtsZ2-1 gene is also high in male flowers,indicating that cucumber FtsZs genes play a role in the growth and development of leaves,flowers,and ovaries;additionally,in the process of responding to coercion,the CsFtsZ2-1 gene can respond to salt stress,CsFtsZ1,CsFtsZ2-1,and CsFtsZ2-2 can respond to cold stress,and CsFtsZ1 and CsFtsZ2-1 can respond to powdery mildew pathogen infection.The research results provide a reference and basis for further study of the role and mechanism of FtsZs genes in cucumber.

关 键 词：黄瓜 FtsZs基因 生物信息学分析 非生物胁迫反应 生物胁迫反应 

分 类 号：S642.2[农业科学—蔬菜学]