山茶花及灵芝精萃物保湿霜对人体皮肤微生态的影响  

Effects of Moisturizers Containing Camellia Extract and Ganoderma Extract on Human Skin Micro-Ecology

作　　者：冯静 周少璐 黄健聪 文霞 苏皑庭 黄迪 施庆珊 谢小保 FENG Jing;ZHOU Shaolu;HUANG Jiancong;WEN Xia;SU Aiting;HUANG Di;SHI Qingshan;XIE Xiaobao(Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application,State Key Laboratory of Applied Microbiology Southern China,Institute of Microbiology,Guangdong Academy of Sciences,Guangdong Detection Center of Microbiology,Guangzhou,Guangdong 510070,China)

机构地区：[1]广东省科学院微生物研究所,广东省微生物分析检测中心,华南应用微生物国家重点实验室,广东省菌种保藏与应用重点实验室,广东广州510070

出　　处：《中国洗涤用品工业》2024年第10期36-49,共14页China Cleaning Industry

基　　金：广东省科学院打造综合产业技术创新中心行动资金项目(2022GDASZH-2022010101)。

摘　　要：为探讨天然植物源提取物护肤品对人体皮肤微生态的影响,以山茶花及灵芝精萃物保湿霜为供试物,采用16S rRNA及ITS rRNA基因高通量测序方法,结合电容法测定皮肤角质层含水量,揭示了保湿霜使用前及使用后14 d和28 d时皮肤微生物群落多样性及种群结构的变化过程与内在原因。研究发现,山茶花及灵芝精萃物保湿霜的使用增加了皮肤细菌群落的多样性,但同时也降低了真菌种群的物种数量。D0、D14及D28组样本分别聚类在不同区域且具备明显区分度。两种保湿霜的使用未改变皮肤微生物在门水平上的群落构成,使用前后细菌及真菌优势门相同。在属水平上,山茶花及灵芝精萃物保湿霜的使用显著增加了细菌中葡萄球菌属和表皮杆状菌属的相对丰度,但真菌群落的相对丰度呈无序变化。此外,两种保湿霜的使用均大幅提升了皮肤角质层的水分含量。结果表明,山茶花及灵芝精萃物保湿霜具有补水保湿功效,可有效调节并改善皮肤菌群的微生态平衡。To investigate the impact of natural plant extracts from Camellia japonica and Ganoderma lucidum on human skin micro-ecology,aiming to provide a theoretical foundation for the development and application of natural plant-based skincare products.Two moisturizers containing extracts derived from Camellia japonica and Ganoderma lucidum were employed in this study as the auxiliary materials.By using 16S rRNA and ITS rRNA high-throughput sequencing technology,in conjunction with capacitance-based determination of skin cuticle moisture content,we revealed the changes in diversity and population structure of skin microbial communities prior to moisturizers application,as well as at 14-day and 28-day intervals post-application.The utilization of moisturizers resulted in an increased diversity of bacterial communities,while simultaneously reducing the fungal species count.The samples from the D0,D14,and D28 groups exhibited distinct clustering patterns with evident discrimination.However,at the phylum level,there were no alterations observed in the community composition of skin microorganisms following the application of these two moisturizers.Firmicutes,Bacteroidetes,Proteobacteria,and Actinobacteria remained as dominant bacteria both before and after using the moisturizers;likewise Ascomycetes and Basidiomycetes persisted as dominant fungi.At the genus level,Staphylococcus and Epidermotrichum showed a significant increase in relative abundance among bacteria upon utilizing of moisturizers;however,changes in relative abundance among fungal genera appeared disordered.Furthermore,employing these two moisturizers greatly enhanced moisture content within the stratum corneum.The two moisturizers containing extracts from Camellia and Ganoderma possess the capability to effectively hydrate and replenish the skin,while also regulating and enhancing the micro-ecological balance of cutaneous flora.

关 键 词：保湿霜 皮肤 微生态 高通量测序 角质层含水量 

分 类 号：TQ658[化学工程—精细化工]