Repeated release of cerium oxide nanoparticles altered algal responses:Growth,photosynthesis,and photosynthetic gene expression  

作　　者：Saibo Liu Jingheng Han Xiaowu Ma Xiaoshan Zhu Han Qu Guorong Xin Xiaochen Huang 

机构地区：[1]State Key Lab of Biocontrol,Guangdong Provincial Key Laboratory of Plant Resources,School of Agriculture and Biotechnology,Shenzhen Campus of Sun Yat-sen University,Shenzhen 518107,China [2]College of Ecology and Environment,Hainan University,Haikou 570228,China [3]College of Environment and Ecology,Key Laboratory of the Three Gorges Reservoir Region's Eco-Environment,Ministry of Education,Chongqing University,Chongqing 400045,China

出　　处：《Eco-Environment & Health》2024年第3期290-299,共10页生态环境与健康（英文）

基　　金：provided by grants from the National Natural Science Foundation of China(42007285,42077227);Shenzhen Science and Technology Program(Grant Nos.RCBS20210706092344024 and 202206193000001,20220816102553004);Basic and Applied Basic Research Foundation of Guangdong Province(No.2023A1515010564).

摘　　要：The expanding production of engineered nanomaterials(ENMs)can eventually cause their increased release into and presence in aquatic ecosystems,potentially threatening the health of aquatic organisms and the stability of the ecological environment.Generally,ENMs are repeatedly released into real-world aquatic environments in relatively low concentrations,potentially affecting photosynthesis in primary producers such as algae.However,knowledge regarding the effects of repeated exposure to ENMs on algal photosynthesis is still lacking.Herein,the physiological responses of the freshwater algae Chlorella vulgaris following single and repeated exposures to cerium oxide nanoparticles(CeO2 NPs)were investigated at 10 mg/L,with a focus on photosynthesis.The results showed that repeated exposures triggered increased photosynthetic pigment contents,oxidative stress levels,decreased photosynthetic performance,and lower biomass in C.vulgaris compared to a single exposure.Photosynthesis-related genes(i.e.,petA,petB,psaA,atpB,and rbcL)were found to be upregulated following repeated exposures.Particularly for petB,repeated rather than single exposure treatment significantly upregulated its expression levels by 2.92–10.24-fold compared to unexposed controls.Furthermore,increased exposure times could aggravate the interaction between CeO2 NPs and algae,elevating 8.13%,12.13%,and 20.51%Ce distribution on the algal cell surface or intracellularly,compared to a single exposure.This study is the first to investigate the effects of ENM exposure times on algal photosynthesis,providing new insights into the assessment of the risks these materials pose to real-world aquatic environments.

关 键 词：Cerium oxide nanoparticles Environmental risks Repeated exposures ALGAE PHOTOSYNTHESIS Gene expression level 

分 类 号：X52[环境科学与工程—环境工程] TB383[一般工业技术—材料科学与工程]