基于UPLC-QTOF-MS/MS的卷柏化学成分分析  

Chemical constituents of Selaginellae Herba based on UPLC-QTOF-MS/MS

作　　者：温静 阎新佳 WEN Jing;YAN Xin-jia(School of Pharmacy,Sichuan College of Traditional Chinese Medicine;Mianyang Key Laboratory of Development and Utilization of Chinese Medicine Resources,Mianyang 621000,China;Institute of Qinghai-Tibetan Plateau,Southwest Minzu University,Chengdu 610041,China;Sichuan Provincial Qiang-Yi Medicinal Resources Protection and Utilization Technology Engineering Laboratory;Tibetan Plateau Ethnic Medicinal Resources Protection and Utilization Key Laboratory of National Ethnic Affairs Commission of the People′s Republic of China,Chengdu 610225,China)

机构地区：[1]四川中医药高等专科学校药学院 [2]绵阳市中药资源开发利用重点实验室,绵阳621000 [3]西南民族大学青藏高原研究院,成都610041 [4]四川省羌彝药用资源保护与利用技术工程实验室 [5]青藏高原民族药用资源保护与利用国家民委重点实验室,成都610225

出　　处：《天然产物研究与开发》2024年第10期1660-1669,共10页Natural Product Research and Development

基　　金：四川省自然科学基金(面上)(2024NSFSC0706);西南民族大学科研启动金项目(RQD2022050);四川中医药高等专科学校高层次人才科研启动经费资助项目(24ZRBS05)。

摘　　要：利用超高效液相色谱-四级杆飞行时间串联质谱法(UPLC-QTOF-MS/MS)对中药卷柏的化学成分进行快速分析及鉴定。采用Agilent Poroshell 120 SB-Aq(2.7μm,2.1 mm×100 mm)色谱柱,以乙腈-0.1%甲酸水溶液为流动相,梯度洗脱,流速为0.4 mL/min,进样量为3μL;选择电喷雾离子源分别在正、负离子模式下扫描分析,通过保留时间、精确相对分子质量和二级质谱裂解碎片,对所得成分的主要色谱峰进行鉴定。结果显示从卷柏中共鉴定出54个化学成分,主要包括双黄酮及黄酮类15个、炔酚类12个、酚酸类10个、苯丙素类5个、甾体类2个、萜类2个、氨基酸及肽类2个、酯类2个和其他类4个,并首次探讨炔酚类成分的裂解规律。该研究建立的UPLC-QTOF-MS/MS方法可以快速准确且较全面地分析鉴定卷柏中的化学成分,可为其质量控制及药效物质基础研究提供科学依据。Ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry(UPLC-QTOF-MS/MS)was employed to rapidly analyze and identify the chemical constituents of Selaginellae Herba.An Agilent Poroshell 120 SB-Aq(2.7μm,2.1 mm×100 mm)chromatographic column was used with acetonitrile-0.1%formic acid aqueous solution as the mobile phase in gradient elution,at a flow rate of 0.4 mL/min and an injection volume of 3μL.The electrospray ion source was selected to scan analyze in both positive and negative ion modes.The main chromatographic peaks of the obtained constituents were identified through retention time,accurate relative molecular mass,and secondary mass spectrometry fragmentation.A total of 54 compounds were identified in Selaginellae Herba,including 15 biflavonoids and flavonoids,12 selaginellins,ten phenolic acids,five benzenepropanoids,two steroids,two terpenoids,two amino acids and peptides,two esters,and four other categories.The UPLC-QTOF-MS/MS method established in this study can rapidly,accurately,and comprehensively analyze and identify the chemical constituents in Selaginellae Herba,providing a scientific basis for its quality control and pharmacological substance basis research.

关 键 词：卷柏 UPLC-QTOF-MS/MS 双黄酮类 炔酚类 

分 类 号：R284.1[医药卫生—中药学]