金果榄二萜类成分及其抗炎活性研究  

Diterpenoids from Tinosporae Radix and their anti-inflammatory activity

作　　者：吕福杰 范在举 李琦 邓爱霞[2] LYU Fu-jie;FAN Zai-ju;LI Qi;DENG Ai-xia(Preparation Center of Liaocheng Traditional Chinese Medicine Hospital;School of Materials Science and Engineering,Liaocheng University,Liaocheng 252000,China)

机构地区：[1]聊城市中医医院制剂中心 [2]聊城大学材料科学与工程学院,聊城252000

出　　处：《天然产物研究与开发》2024年第10期1707-1713,共7页Natural Product Research and Development

基　　金：山东省自然科学基金(ZR2021ME126)。

摘　　要：研究金果榄的二萜类成分及其抗炎活性。采用多种色谱技术对金果榄乙醇提取物进行分离纯化,通过质谱、核磁、圆二色谱及单晶衍射等方法对化合物结构进行鉴定,并以Griess法测定化合物对脂多糖诱导的小鼠小胶质BV2细胞产生一氧化氮(NO)的抑制活性。从金果榄乙醇提取物乙酸乙酯萃取部分分离得到了七个克罗烷型二萜类化合物,分别为tinocapill A(1)、tinocapill B(2)、crispene A(3)、floridolide B(4)、古伦宾(5)、tinosporol B(6)、borapetol A(7)。其中化合物1和2为新化合物,活性结果表明,化合物2和6能够显著抑制LPS诱导小鼠BV2细胞释放NO(IC 50值分别为9.6和5.8μmol/L),具有潜在的抗炎活性。This study aims to investigate the diterpenoids of Tinosporae Radix and their anti-inflammatory activity.Various chromatographic techniques were used for isolation of the ethanol extract of Tinosporae Radix.The structures of the compounds were identified using MS,NMR,CD,and single X-ray crystal diffraction.The Griess method was used to determine the inhibitory activity of the compounds on the production of nitric oxide(NO)in lipopolysaccharide-induced BV2 cells.Seven clerodane diterpenoids were isolated from the EtOAc part of the ethanol extract of Tinosporae Radix,including tinocapill A(1),tinocapill B(2),crispene A(3),floridolide B(4),columbin(5),tinosporol B(6)and borapetol A(7).Compounds 1 and 2 were new compounds,while the activity results indicated that compounds 2 and 6 could significantly inhibit LPS-induced NO release in murine BV2 cells(with IC 50 values of 9.6 and 5.8μmol/L,respectively),showing potential anti-inflammatory activity.

关 键 词：金果榄 提取分离 二萜 克罗烷 抗炎 

分 类 号：R932[医药卫生—生药学]