基于不同遗传群体的大豆分枝数QTL定位分析  

Mapping QTL for soybean branch number based on different genetic population

作　　者：朱晨博 张东梅 孙明明[3] 刘淼[4] 袁明[5] 贾晓轲 赵艳强 张艳婷 杨明亮[1] 陈庆山[1] ZHU Chen-bo;ZHANG Dong-mei;SUN Ming-ming;LIU-Miao;YUAN-Ming;JIA Xiao-ke;ZHAO Yan-qiang;ZHANG Yan-ting;YANG Ming-liang;CHEN Qing-shan(Northeast Agricultural University,Harbin 150030,China;Heilongjiang Bayi Agricultural University,Daqing 163319,China;Soybean Research Institute of Heilongjiang Academy of Agricultural Sciences,Harbin 150086,Chi-na;Cultivation Institute of Heilongjiang Academy of Agricultural Sciences,Harbin 150086,China;Qiqihaer Branch of Heilongjiang Academy of Agricultural Sciences,Qiqihaer 161006,China)

机构地区：[1]东北农业大学,黑龙江哈尔滨150030 [2]黑龙江省八一农垦大学,黑龙江大庆163319 [3]黑龙江省农业科学院大豆研究所,黑龙江哈尔滨150086 [4]黑龙江省农业科学院耕作栽培研究所,黑龙江哈尔滨150086 [5]黑龙江省农业科学院齐齐哈尔分院,黑龙江齐齐哈尔161006

出　　处：《中国油料作物学报》2024年第5期1017-1028,共12页Chinese Journal of Oil Crop Sciences

基　　金：国家重点研发计划课题(2021YFD120160204,2021YFD1201103-01-02)。

摘　　要：分枝数是大豆重要的农艺性状,与大豆株型结构、产量潜力和适应性密切相关。为挖掘大豆分枝数稳定遗传位点,本研究以多分枝大豆Charleston和主茎型大豆东农594为亲本构建的148份重组自交系群体(RILs)和以主茎型大豆绥农14和多分枝野生大豆ZYD00006为亲本构建的213份染色体片段代换系(CSSLs)2个遗传群体为试验材料,采用ICIMapping软件中的完备区间作图法(inclusive composite interval mapping,ICIM)和Win-QTL-Cart 2.5软件中的复合区间作图法(composite interval mapping,CIM),对2018-2021年两个遗传群体的分枝数表型数据进行QTL分析,对QTL置信区间内的候选基因进行挖掘。共检测到17个与大豆分枝数性状相关的QTL位点,其中qBNA2_1和qBNK_1在不同年份和不同群体中稳定出现,分布在第8和9号染色体。根据基因注释等信息对两个QTL区间内的候选基因进行筛选,并通过qRT-PCR预测Glyma.08G053700、Glyma.08G068200、Glyma.08G082400和Glyma.09G167100为调控分枝数的候选基因。本研究结果有助于探明大豆分枝数形成的遗传机制,为大豆理想株型研究提供候选基因与材料支撑。Branch number is an important agronomic trait of soybean,closely associated with the architecture,yield potential and adaptability.To mining the stable genetic loci of soybean branch number,148 recombinant in-bred lines(RILs)population crossed by Charleston(high-branched variety)and Dongnong 594(main stem variety),and 213 chromosome segment substitution lines(CSSL)population crossed by Suinong14(main stem variety)and ZYD00006(high-branched wild variety)were used as materials.With two genetic populations,we used inclusive composite interval mapping(ICIM)method in ICIMapping software and composite interval mapping(CIM)method in Win-QTL-Cart 2.5 software for mapping the QTLs of soybean branch number trait from 2018 to 2021,and the candidate genes from the QTL interval were screened.17 QTLs associated with branch number were detected in the RIL and CSSL population,among which qBNA2_1 and qBNK_1 were repeatedly detected on chromosome 8 and 9 in different years and population.According to gene annotation and qRT-PCR,Glyma.08G053700,Gly-ma.08G068200,Glyma.08G082400 and Glyma.09G167100 were predicted as candidate genes regulating soybean branch number from two stable QTLs interval.These results are helpful to understand the genetic mechanism of branch number formation and provide candidate genes and materials for studying ideal plant type of soybean.

关 键 词：大豆 重组自交系(RIL) 染色体片段代换系(CSSL) 分枝数 QTL 候选基因 

分 类 号：S565.1[农业科学—作物学]