基于SSR标记的毛豆种质资源遗传多样性分析  

Genetic diversity analysis of vegetable soybean germplasm resources based on SSR markers

作　　者：向艳涛 刘昌燕[2] 韩雪松 李莉[2] 孙龙清 陈宏伟[2] 沙爱华[1] XIANG Yan-tao;LIU Chang-yan;HAN Xue-song;LI Li;SUN Long-qing;CHEN Hong-wei;SHA Ai-hua(Key Laboratory of Sustainable Crop Production in the Middle Reaches of the YangtzeRiver(Co-conconsturction by Ministry and Province),Ministry of Agriculture and Rural Affairs,College of Agriculture,Yangtze University,Jing-zhou 434025,China;Institute of Grain Crops,Hubei Academy of Agricultural Sciences/Hubei Provincial Key Labo-ratory of Grain Crop Germplasm Innovation and Genetic Improvement/Key Laboratory of Crop Molecular Breeding,Ministry of Agriculture and Rural Affairs,Wuhan 430064,China)

机构地区：[1]农业农村部长江中游作物绿色高效生产重点实验室(部省共建),长江大学农学院,湖北荆州434025 [2]湖北省农业科学院粮食作物研究所/粮食作物种质创新与遗传改良湖北省重点实验室/农业农村部作物分子育种重点实验室,湖北武汉430064

出　　处：《中国油料作物学报》2024年第5期1029-1039,共11页Chinese Journal of Oil Crop Sciences

基　　金：湖北省重点研发计划项目(2022BBA0036);国家现代农业产业技术体系建设项目(CARS-08);湖北省农业科技创新中心项目(2021-620-000-001-01);湖北省农科院青年拔尖人才项目。

摘　　要：为了解不同毛豆种质资源的遗传基础和群体的亲缘关系,本研究选用212对SSR标记对来自于中国(包括台湾地区、湖北、江西、江苏、辽宁、安徽)和日本的84份毛豆种质材料进行了基因型分析,根据Nei’s遗传距离和非加权配对法(UPGMA)进行聚类分析,以structure软件的混合模型聚类法推断材料构成群体的遗传结构。结果表明:SSR引物在群体材料中扩增出的等位位点数为1-6个,平均每对引物为2.08个;有效等位基因1~3.74之间,平均为1.6个;基因多样性平均值为0.29,PIC平均值为0.24。聚类分析将84份材料分为4个组群,主成分分析的二维和三维主坐标分析图验证了聚类分析结果。群体结构分析显示,当K=4时,ΔK出现明显的峰值,说明最佳群体组群数为4个,该结果与聚类分析结果也基本一致;84份材料中有74份Q值大于0.7,说明该群体毛豆种质资源群体内遗传多样性不够丰富,遗传背景单一。因此,需重视对遗传背景差异大、亲缘关系远的种质资源的发掘利用,加强种质材料共享和交流,促进毛豆育种事业的发展。This study aimed to understand the genetic basis of different vegetable soybean germplasm resourc-es and the kinship of populations,212 pairs of SSR markers were selected to carry out genotypic analysis on 84 soy-bean germplasm materials from China(including Taiwan region,Hubei,Jiangxi,Jiangsu,Liaoning and Anhui)and Japan.Furthermore,Cluster analysis was conducted according to Nei´s genetic distance and unweighted matching method(UPGMA),And the hybrid model clustering method of structure software was used to infer the genetic structure of the material composition population.The results showed that the number of alleles amplified by SSR primers in the population material was 1-6,with an average of 2.08 per primer pair;Effective alleles range from 1-3.74,1.6 on av-erage;The average value of gene diversity is 0.29,and the average value of PIC was 0.24.The 84 materials were di-vided into 4 groups by cluster analysis.The results of cluster analysis were verified by two-dimensional and three-dimensional principal coordinate analysis diagrams of principal component analysis.The population structure analysis showed that when K=4,ΔK showed an obvious peak,indicating that the optimal number of groups was 4,which was basically consistent with the results of cluster analysis.The Q values of 74 of the 84 samples were higher than 0.7,indicating that the genetic diversity of vegetable soybean germplasm resources in this population was not rich enough and the genetic background was single.Therefore,we need to pay attention to the discovery and utiliza-tion of germplasm resources with large differences in genetic background and distant relatives,strengthen the shar-ing and exchange of germplasm materials,and promote the development of vegetable soybean breeding.

关 键 词：毛豆 SSR标记 遗传多样性分析 群体结构分析 

分 类 号：S565.1[农业科学—作物学]