GmYUC12a对大豆侧根发育和抗旱性的调控作用  

Regulatory effect of soybean GmYUC12a on lateral root development and drought resistance in soybean

作　　者：齐玉军[1] 邢兴华[1] 李思梦 徐泽俊[1] 童飞 王好让 江海东[2] 王幸[1] QI Yu-jun;XING Xing-hua;LI Si-meng;XU Ze-jun;TONG Fei;WANG Hao-rang;JIANG Hai-dong;WANG Xing(Xuzhou Institute of Agricultural Sciences of Xu-huai Region of Jiangsu,Xuzhou 221131,China;College of Agriculture/Key Laboratory of Crop Physiology Ecology and Production Management,Ministry of Agriculture and Rural Affairs/Jiangsu Collaborative Innovation Center for Modern Crop Production,Nanjing Agricultural University,Nanjing 210095,China)

机构地区：[1]江苏徐淮地区徐州农业科学研究所,江苏徐州221131 [2]南京农业大学农学院/农业农村部作物生理生态与生产管理重点实验室/江苏省现代作物生产协同创新中心,江苏南京210095

出　　处：《中国油料作物学报》2024年第5期1068-1078,共11页Chinese Journal of Oil Crop Sciences

基　　金：徐州市重点研发计划项目(KC22072);徐州市农业科学院科研基金项目(XM2020001)。

摘　　要：侧根对作物抗旱具有重要作用,明确Gm YUC12a在大豆侧根生长发育和抗旱中的作用,为探究大豆侧根发育响应干旱的作用机制奠定基础。本文首先分析了徐豆18和徐9302在5%(m/V)聚乙二醇6000模拟干旱胁迫下根系形态特征、根系内源生长素(IAA)含量、IAA合成和信号转导相关基因表达量以及叶片净光合速率(P_(n))和相对含水量(RWC)的变化。干旱胁迫下根系IAA含量、Gm YUC12a、Gm TIR1A和Gm AFB3A表达量变化趋势与侧根数、侧根长、总根长一致,徐9302各项指标均较对照增加,徐豆18则相反,因而徐豆18叶片P_(n)和RWC下降幅度较大。通过发根农杆菌侵染徐9302下胚轴,获得转基因毛状根嵌合植株。干旱胁迫下Gm YUC12a过表达毛状根嵌合体根系IAA含量、Gm TIR1A、Gm AFB3A表达水平、叶片P_(n)和RWC以及侧根数量和长度显著高于空载毛状根嵌合体。与之不同,干旱胁迫下Gm TIR1A、Gm AFB3A过表达毛状根嵌合体侧根数量和长度较空载毛状根复合体无变化,而GmTIR1A和Gm AFB3A同时过表达毛状根嵌合体侧根数量和长度显著增加。可见,干旱胁迫下Gm YUC12a诱导GmTIR1A和Gm AFB3A表达,进而Gm TIR1A和Gm AFB3A协同调控侧根生长发育,提高植株抗旱性。Lateral root(LR)plays an important role in plant drought resistance.This study was carried out to explore role of GmYUC12a in LR development under drought stress and lay a foundation for analysis of the response mechanism of soybean LR to drought stress.The changes of morphological characteristics,indole-3-acetic acid content and expression levels of several genes involved in IAA biosynthesis and signal transduction of roots and net photosynthetic rate(P_(n))and relative water content(RWC)of leaves have been analyzed in Xudou 18 and Xu 9302 exposed to drought stress induced by 5%(m/V)polyethylene glycol 6000 treatment.Drought stressed improved LR number and length,total root length and IAA content,GmYUC12a,GmTIR1A and GmAFB3A expression levels in roots of Xu 9302,while the opposite effects on Xudou 18 were observed,which triggering a smaller drought-in-duced decrease in P_(n) and RWC in Xu 9302.The transgenic Xu 9302 hairy root complexes were generated by Agro-bacterium-mediated transformation method.Overexpression analyses showed that GmYUC12a caused an increase in LR number and length,total root length,IAA content and GmYUC12a,GmTIR1A and GmAFB3A expression levels in hairy roots and P_(n) and RWC in leaves of stressed Xu 9302 hairy root complexes.However,LR number and length did not change in stressed GmTIR1A-overexpressing and GmAFB3A-overexpressing hairy root complexes,as com-pared with stressed empty-vector hairy root complexes,while they increased in stressed complexes with hairy roots simultaneous overexpressing GmTIR1A and GmAFB3A.Thus,GmYUC12a up-regulated GmTIR1A and AFB3A which cooperated to induce LR development under drought stress,ultimately improving drought resistance in soy-bean.

关 键 词：大豆 干旱 侧根 GmYUC12a GmTIR1A GmAFB3A 

分 类 号：S565.1[农业科学—作物学]