Three-dimensional bioprinting biphasic multicellular living scaffold facilitates osteochondral defect regeneration  

作　　者：Xingge Yu Mazaher Gholipourmalekabadi Xudong Wang Changyong Yuan Kaili Lin 

机构地区：[1]Department of Oral and Cranio-Maxillofacial Science,Shanghai Ninth People's Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai,China [2]College of Stomatology,Shanghai Jiao Tong University,Shanghai,China [3]National Center for Stomatology,Shanghai,China [4]National Clinical Research Center for Oral Diseases,Shanghai,China [5]Shanghai Key Laboratory of Stomatology,Shanghai,China [6]Research Unit of Oral and Maxillofacial Regenerative Medicine,Chinese Academy of Medical Sciences,Shanghai,China [7]Cellular and Molecular Research Center,Iran University of Medical Sciences,Tehran,Iran [8]Department of Medical Biotechnology,Faculty of Allied Medicine,Iran University of Medical Sciences,Tehran,Iran [9]Affiliated Stomatological Hospital of Xuzhou Medical University,Xuzhou,Jiangsu,China [10]School of Stomatology,Xuzhou Medical University,Xuzhou,Jiangsu,China

出　　处：《Interdisciplinary Materials》2024年第5期738-756,共19页交叉学科材料（英文）

基　　金：National Natural Science Foundation of China,Grant/Award Numbers:82072396,32271379;CAMS Innovation Fund for Medical Sciences,Grant/Award Numbers:CIFMS,2019-I2M-5-037;Shanghai's Top Priority Research Center,Grant/Award Number:2022ZZ01017;Interdisciplinary Program of Shanghai Jiao Tong University,Grant/Award Number:YG2021ZD12;Science and Technology Commission of Shanghai Municipality,Grant/Award Number:21490711700;Science and Technology Project of Xuzhou Health Commission,Grant/Award Number:XWKYHT20230077。

摘　　要：Due to tissue lineage variances and the anisotropic physiological character-istics,regenerating complex osteochondral tissues(cartilage and subchondral bone)remains a great challenge,which is primarily due to the distinct requirements for cartilage and subchondral bone regeneration.For cartilage regeneration,a significant amount of newly generated chondrocytes is required while maintaining their phenotype.Conversely,bone regeneration necessitates inducing stem cells to differentiate into osteoblasts.Additionally,the construction of the osteochondral interface is crucial.In this study,we fabricated a biphasic multicellular bioprinted scaffold mimicking natural osteochondral tissue employing three-dimensional(3D)bioprinting technol-ogy.Briefly,gelatin-methacryloyl(GelMA)loaded with articular chondrocytes and bone marrow mesenchymal stem cells(ACs/BMSCs),serving as the cartilage layer,preserved the phenotype of ACs and promoted the differentia-tion of BMSCs into chondrocytes through the interaction between ACs and BMSCs,thereby facilitating cartilage regeneration.GelMA/strontium-substituted xonotlite(Sr-CSH)loaded with BMSCs,serving as the subchondral bone layer,regulated the differentiation of BMSCs into osteoblasts and enhanced the secretion of cartilage matrix by ACs in the cartilage layer through the slow release of bioactive ions from Sr-CSH.Additionally,GelMA,serving as the matrix material,contributed to the reconstruction of the osteochondral interface.Ultimately,this biphasic multicellular bioprinted scaffold demonstrated satisfactory simultaneous regeneration of osteochondral defects.In this study,a promising strategy for the application of 3D bioprinting technology in complex tissue regeneration was proposed.

关 键 词：3D bioprinting biphasic scaffolds interface reconstruction MULTICELLULARITY osteochondral defects 

分 类 号：TP391.73[自动化与计算机技术—计算机应用技术] R68[自动化与计算机技术—计算机科学与技术]