小麦赤霉病菌产毒条件及毒素对斑马鱼的雌激素响应研究  

Study on the toxin production conditions of zearalenone and the estrogen response of toxin to zebrafish

作　　者：李瑞瑞 陶怡洁 郭会 陈武炼 刘家贝 许欣悦 杨甜甜 徐文平[1] LI Ruirui;TAO Yijie;GUO Hui;CHEN Wulian;LIU Jiabei;XU Xinyue;YANG Tiantian;XU Wenping(East China University of Science and Technology,Shanghai 200237,China;Shanghai Anpu Laboratory Technology Co.,Ltd.,Shanghai 201609,China)

机构地区：[1]华东理工大学药学院,上海200237 [2]上海安谱实验科技股份有限公司,上海201609

出　　处：《世界农药》2024年第10期37-46,共10页World Pesticide

基　　金：大学生创新创业训练计划(X202310251133)。

摘　　要：旨在优化玉米赤霉烯酮(ZEN)的产毒条件,并探讨ZEN对斑马鱼雌激素响应的影响。通过更换培养基配方并使用高效液相色谱技术,发现提供生长所需无机盐的环境有利于ZEN的积累。在毒性试验中,斑马鱼胚胎暴露于不同浓度ZEN(0、0.1、0.2、0.4μg/m L)后,斑马鱼心率随浓度升高而降低,48、72 h胚胎孵化率显著抑制,并且出现脊柱弯曲、尾部弯曲和卵黄囊水肿等畸形。同时,暴露于ZEN 72 h的胚胎中雌激素表达在72 h暴露后显著抑制,且呈浓度梯度效应。进一步试验显示,0.2μg/L ZEN暴露7 d显著影响成年斑马鱼的受精率和胚胎存活率,且对雌鱼影响大于雄鱼。结果明确了ZEN的积累与培养基中无机盐的添加有关,表明ZEN对于斑马鱼的胚胎发育具有显著毒性,且对斑马鱼成鱼的生殖毒性存在明显的性别偏向,可为ZEN的脱毒、检测及环境管理提供科学依据。The aim of this study was to optimize the toxicity of zearalenone(ZEN) and to explore the effect of ZEN on the estrogen response of zebrafish.By changing the medium formula and using high performance liquid chromatography(HPLC),it was found that an environment that provides inorganic salts required for growth is conducive to the accumulation of ZEN.In the toxicity test,after zebrafish embryos were exposed to different concentrations of ZEN(0,0.1,0.2,0.4 μg/mL),the heart rate of zebrafish decreased with increasing concentration,the hatching rate of embryos at 48 h and 72 h was significantly inhibited,and deformities such as spinal curvature,tail curvature,and yolk sac edema occurred.At the same time,the expression of estrogen in embryos exposed to ZEN for 72 h was significantly inhibited after 72 h of exposure,and it showed a concentration gradient effect.Further experiments showed that exposure to 0.2 μg/L ZEN for 7 days significantly affected the fertilization rate and embryonic survival rate of adult zebrafish,and the effect on female fish was greater than that on male fish.The results clearly showed that the accumulation of ZEN was related to the addition of inorganic salts in the culture medium,indicating that ZEN was significantly toxic to the embryonic development of zebrafish and that its reproductive toxicity to adult zebrafish was significantly gender-biased,which could provide a scientific basis for the detoxification,detection and environmental management of ZEN.

关 键 词：玉米赤霉烯酮 雌激素效应 产毒条件优化 斑马鱼 

分 类 号：TQ455[化学工程—农药化工]