脱离低压低氧环境对高原肺动脉高压大鼠肺血管重建和TNF-α表达的影响  

Effects of leaving hypobaric and hypoxic environment on pulmonary vascular reconstruction and TNF-α expression in rats with high altitude pulmonary hypertension

作　　者：石海霞[1] 赵永峰 李肃[1] 吴海涛[1] 侯明[1] SHI Haixia;ZHAO Yongfeng;LI Su;WU Haitao;HOU Ming(Department of Emergency,the Affiliated Hospital of Qinghai University,Xining 810001,Qinghai,China;Department of Ultrasound,the Affiliated Hospital of Qinghai University,Xining 810001,Qinghai,China)

机构地区：[1]青海大学附属医院急诊科,青海西宁810001 [2]青海大学附属医院超声科,青海西宁810001

出　　处：《中南医学科学杂志》2024年第5期721-724,785,共5页Medical Science Journal of Central South China

基　　金：青海省卫生健康委课题(2019-wjzdx-56)。

摘　　要：目的 探究脱离低压低氧环境对高原肺动脉高压大鼠肺血管重建和肿瘤坏死因子-α(TNF-α)表达的影响。方法 取120只Wistar大鼠随机分为对照组、高海拔组(10、20、30天)、低海拔组(10、20、30、90天)。测定各组肺动脉压力和右心室肥厚指标,采用HE染色观察大鼠肺组织病理变化,评估肺血管重建程度[肺小动脉管壁厚度/外径百分比(WT%)和管壁面积/总面积百分比(WA%)],免疫组化法检测肺组织切片中TNF-α的表达。结果 与对照组比较,高海拔组大鼠平均肺动脉压(mPAP)、右心室肥厚指数(RVHI)、WT%、WA%、肺组织TNF-α表达均升高;与高海拔30天组比较,低海拔组大鼠mPAP、RVHI、WT%、WA%、肺组织TNF-α表达均降低;且随时间延长,变化更为显著(P<0.05)。随造模时间延长,肺血管管壁厚度呈逐步增厚,管腔逐步变小;以高海拔地区造模30天为低海拔组参考,随着返低海拔地区时间延长,肺血管管壁厚度呈逐步变薄,管腔逐步变大。结论 脱离低压低氧环境后,高原肺动脉高压大鼠右心室肥厚和肺血管重建改善,肺组织TNF-α表达降低。Aim To explore the effects of leaving hypobaric and hypoxic environment on pulmonary vascular reconstruction and tumor necrosis factor α(TNF-α) expression in rats with high altitude pulmonary hypertension.Methods A total of 120 Wistar rats were randomly divided into control group,high-altitude group(10 d,20 d,30 d) and low-altitude group(10 d,20 d,30 d,90 d).The pulmonary artery pressure and right ventricular hypertrophy indexes were detected.The pathological changes of lung tissues were observed by HE staining,the degree of pulmonary vascular reconstruction [the percentage of pulmonary arteriole wall thickness to outer diameter(WT%),and the percentage of tube wall area to total area(WA%)] was evaluated,and expression of TNF-α in lung tissue sections was detected by immunohistochemistry.Results Compared with control group,mean pulmonary artery pressure(mPAP),right ventricular hypertrophy index(RVHI),WT%,WA% and expression of TNF-α were increased in high-altitude group.Compared with high-altitude 30d group,mPAP,RVHI,WT%,WA% and expression of TNF-α were decreased in low-altitude group.With time extension,the above changes were more significant(P<0.05).With the extension of molding time,thickness of pulmonary vascular wall was gradually thickened,and lumen was gradually decreased.By using 30d molding in high altitude area as reference to low altitude group,with the extension of returning low altitude area time,thickness of pulmonary vascular wall was gradually thinned,lumen was gradually increased(P<0.05).Conclusion After leaving hypobaric and hypoxic environment,right ventricular hypertrophy and pulmonary vascular reconstruction are improved,and expression of TNF-α in lung tissues is decreased in rats with high altitude pulmonary hypertension.

关 键 词：高原肺动脉高压大鼠 低压低氧 肺血管重建 肿瘤坏死因子-Α 

分 类 号：R563[医药卫生—呼吸系统]