维生素D衍生物卡泊三醇对小鼠斑秃治疗有效性及相关炎症因子的影响  

Effects of vitamin D derivative Calciportriol on therapeutic efficacy and related inflammatory factors in mice with alopecia areata

作　　者：赵芬[1] 贺美林[1] 田黎明[1] 胡枫[1] 姜倩[1] 陈龙[1] 田庆均[1] ZHAO Fen;HE Meilin;TIAN Liming;HU Feng;JIANG Qian;CHEN Long;TIAN Qingjun(Department of Dermatology,Wuhan NO.1 Hospital,Wuhan 430022,Hubei,China)

机构地区：[1]武汉市第一医院皮肤科,湖北武汉430022

出　　处：《中南医学科学杂志》2024年第5期735-738,共4页Medical Science Journal of Central South China

基　　金：武汉市卫生健康委课题(WX20D37)。

摘　　要：目的 研究维生素D衍生物卡泊三醇对小鼠斑秃的缓解情况及对相关炎症因子的影响。方法 构建小鼠斑秃模型,将维生素D衍生物卡泊三醇均匀涂抹在小鼠斑秃区域后,观察小鼠斑秃缓解情况;同时,通过免疫荧光染色技术检测斑秃区域皮肤组织CD4^(+)、CD8^(+) T细胞和肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)等细胞因子情况。结果 卡泊三醇处理后小鼠斑秃完全消失,治疗有效率达100%;小鼠毛囊细胞周围与炎症反应相关的CD4^(+)和CD8^(+)T细胞数量降低,TNF-α、IL-6等炎症细胞因子分泌水平下降(P<0.05)。结论 卡泊三醇可通过抑制小鼠毛囊细胞周围与炎症反应相关的CD4^(+)和CD8^(+)T细胞数量以及TNF-α、IL-6等炎症细胞因子分泌而改善小鼠斑秃。Aim To investigate the efficacy of Calciportriol,a vitamin D derivative,on mice with alopecia areata and related inflammatory factors.Methods Mouse models of alopecia areata were constructed.Then,the vitamin D derivative calcipotriol was evenly smeared on the alopecia areata area of the mice.The efficacy of alopecia areata in the mice was observed.Furthermore,immunofluorescence staining was used to detect CD4~+,CD8~+ T cells and related cytokines,such as TNF-α and IL-6,in alopecia areata areas.Results Alopecia areata disappeared completely after treatment with Calciportriol,and the effective rate was 100%.The number of CD4~+ and CD8~+T cells associated with inflammation around mouse hair follicle cells decreased,accompanying with suppressed secretion of inflammatory cytokines such as TNF-α and IL-6(P<0.05).Conclusion Carpotriol can significantly improve alopecia areata in mice by inhibiting the number of CD4~+ and CD8~+T cells and the secretion of TNF-α,IL-6 and other inflammatory cytokines around hair follicle cells.

关 键 词：维生素D衍生物 卡泊三醇 维生素D受体 斑秃炎症因子 

分 类 号：R758.71[医药卫生—皮肤病学与性病学]