牛磺熊去氧胆酸和熊胆粉对神经炎症的保护效果评价  

Protective Effect of Tauroursodeoxycholic Acid and Bear Bile Powder on Neuroinflammation

作　　者：葛紫霏 李瑞莲 未金花[2] 张毓宸 杜昱光[2] 王倬 王冰 GE Zifei;LI Ruilian;WEI Jinhua;ZHANG Yuchen;DU Yuguang;WANG Zhuo;WANG Bing(Medical College,Guangxi University,Nanning 530004,China;Key Laboratory of Biopharmaceutical Preparation and Delivery,Chinese Academy of Sciences,State Key Laboratory of Biochemical Engineering,Institute of Process Engineering,Chinese Academy of Sciences,Beijing 100190,China)

机构地区：[1]广西大学医学院,南宁530004 [2]中国科学院过程工程研究所,生化工程国家重点实验室,生物药制备与递送重点实验室(中国科学院),北京100190

出　　处：《中国生物工程杂志》2024年第9期14-22,共9页China Biotechnology

基　　金：国家自然科学基金面上项目(82173957)资助项目。

摘　　要：熊胆粉是从黑熊胆囊中提取的一种传统中药,牛磺熊去氧胆酸(TUDCA)被认为是熊胆粉中发挥活性的主要成分及重要药物质量标准。但TUDCA在熊胆粉中的含量只占20%~30%,这引起了对熊胆粉其他成分功能活性以及其与TUDCA在生物活性上是否存在差异的疑问。探讨熊胆粉和TUDCA对神经炎症的改善作用,分别用不同浓度的TUDCA和熊胆粉对小胶质细胞BV-2进行预处理,再通过脂多糖(LPS)诱导BV-2的炎症反应,通过qRT-PCR和蛋白质印迹法检测BV-2细胞中炎症因子和相关炎症通路中关键蛋白质表达的变化。在动物实验中,分别用TUDCA和熊胆粉连续灌胃处理C57BL/6J小鼠21天,用LPS连续腹腔注射7天,通过对小鼠脑组织病理切片和炎症因子表达水平的检测评价TUDCA和熊胆粉对小鼠神经炎症的抑制作用。实验结果显示,TUDCA和熊胆粉都能有效抑制神经炎症。在相同剂量作用下,TUDCA和熊胆粉在抑制神经炎症的效力没有显著差异,这表明熊胆粉的其他活性成分可能与TUDCA共同在抑制神经炎症过程中发挥了不可替代的作用。因此,熊胆粉中不同成分的具体功效以及它们之间的协同作用关系值得进一步深入研究。研究不仅拓展了对熊胆粉作用机制的认识,也为开发新型抗神经炎症药物提供了有价值的参考。Bear bile powder(BBP)is a traditional Chinese medicine extracted from the gallbladder of black bears.Tauroursodeoxycholic acid(TUDCA)is considered to be the main active ingredient in BBP and a critical quality standard for the drug.However,TUDCA only accounts for around 20%to 30%of bear bile powder(BBP).The roles and activities of other components in BBP and possible differences in biological activities between TUDCA and BBP remain unclear.This study aims to investigate the effects of bear bile powder and TUDCA on neuroinflammation.In our research,different concentrations of TUDCA and bear bile powder were used to pre-treat BV-2 microglial cells,followed by the induction of an inflammatory response in these cells using lipopolysaccharide(LPS).Changes in the expression of inflammatory factors and key proteins in related inflammatory pathways were monitored using quantitative reverse transcription polymerase chain reaction(qRT-PCR)and Western blot(WB)techniques.Additionally,in animal experiments,C57BL/6J mice were treated with continuous gavage of TUDCA and bear bile powder for 21 days,followed by continuous intraperitoneal injections of LPS for 7 days.The inhibitory effects of TUDCA and bear bile powder on neuroinflammation in mice were evaluated by examining pathological sections of mouse brain tissue and the expression levels of inflammatory factors.The results of both cell and animal experiments indicate that TUDCA and bear bile powder both have an inhibitory effect on neuroinflammation.No significant difference in the anti-inflammatory activity was observed between the same doses of TUDCA and bear bile powder,suggesting that other active components of bear bile powder play an irreplaceable role in conjunction with TUDCA in inhibiting neuroinflammation.Therefore,the specific effects of different components of bear bile powder and their synergistic relationships deserve further investigation.This research not only expands our understanding of the mechanisms of action of bear bile powder,but also provides valuabl

关 键 词：熊胆粉 牛磺熊去氧胆酸 脂多糖神经炎症 

分 类 号：Q42[生物学—神经生物学]