抗体识别表位分析方法研究进展  

Advances in Monoclonal Antibody Epitope Analysis Technology

作　　者：盛柔方 宋飞波 葛胜祥[1,2] 李廷栋[1,2] SHENG Roufang;SONG Feibo;GE Shengxiang;LI Tingdong(National Key Laboratory for the Research and Development of Infectious Disease Vaccines,School of Public Health,Xiamen University,Xiamen 361102,China;National Institute of Diagnostics and Vaccine Development in Infectious Diseases,School of Public Health,Xiamen University,Xiamen 361102,China)

机构地区：[1]厦门大学公共卫生学院实验医学系,传染病疫苗研发全国重点实验室翔安实验室,厦门361102 [2]厦门大学国家传染病诊断试剂与疫苗工程技术研究中心、分子疫苗学和分子诊断学重点实验室、国家医学攻关产教融合创新平台(疫苗研发),厦门361102

出　　处：《中国生物工程杂志》2024年第9期64-72,共9页China Biotechnology

基　　金：国家自然科学基金(82171816)资助项目。

摘　　要：单克隆抗体在生物医学领域中具有广泛应用,其特异性识别抗原的能力对于疾病治疗和疫苗设计至关重要。精确定位单克隆抗体识别表位有助于深入了解抗体与抗原的相互作用机制,可以为抗体药物的设计和优化以及疫苗设计提供重要依据。单克隆抗体表位分析技术的不断发展为抗体应用提供了有力支持。现有的单克隆抗体表位分析技术有丙氨酸扫描、多肽微阵列、结构域互换、X射线晶体衍射技术、冷冻电镜技术等。不同技术各具特色,在选择适合的分析方法时,需要综合考虑实验需求、样本特性及技术可行性等因素。综述了现有的单克隆抗体表位分析技术,比较了不同技术的优缺点,旨在为单克隆抗体表位分析的方法选择提供参考,推动单克隆抗体在生物医学领域的进一步发展。Known for their unparalleled ability to specifically target antigens,monoclonal antibodies have become indispensable in biomedicine.Their precision in recognizing antigens is critical for the treatment of diseases and the development of vaccines.A thorough understanding of the epitopes bound by these antibodies is essential to elucidate the mechanisms underlying antibody-antigen interactions.This knowledge serves as a cornerstone for optimizing the development of antibody-based therapeutics and vaccines.The field has been greatly advanced by the development of techniques for monoclonal antibody epitope analysis,including methods such as alanine scanning,peptide microarrays,domain swapping,X-ray crystallography,and cryo-electron microscopy.This article provides a comprehensive assessment of these techniques,weighing the strengths and limitations of each.Its objective is to guide researchers in selecting the most appropriate approach to monoclonal antibody epitope analysis and to promote further advances in this important area of biomedical research.

关 键 词：单克隆抗体 抗原表位 表位识别技术 

分 类 号：Q511[生物学—生物化学]