鱼藤酮对斜纹夜蛾低毒原因分析及混剂增效作用研究  

Stuty on the cause of low toxicity of rotenone against Spodoptera litura and the synergistic effect of rotenone mixture

作　　者：李伟声 王彦辉[1] 马永林[1] 郭成林[1] 张志祥[2] Li Weisheng;Wang Yanhui;Ma Yonglin;Guo Chenglin;Zhang Zhixiang(Plant Protection Research Institute,Guangxi Academy of Academy of Agricultural Science/Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China,Ministry of Agriculture and Rural Affairs/Guangxi Key Laboratory of Biology for Crop Diseases and Insect Pests,Nanning Guangxi 530007,China;Key Laboratory of Natural Pesticide and Biochemistry,Ministry of Education,Department of Pesticide Science,College of Crop Protection,South China Agriculture University,Guangzhou Guangdong 510642,China)

机构地区：[1]广西农业科学院植物保护研究所/农业农村部华南果蔬绿色防控重点实验室/广西作物病虫害生物学重点实验室,广西南宁530007 [2]天然农药与化学生物学教育部重点实验室/华南农业大学植物保护学院,广东广州510642

出　　处：《中国植保导刊》2024年第8期5-9,共5页China Plant Protection

基　　金：广西农业科学院基本科研业务专项(桂农科2024YP059)。

摘　　要：为探索植物源杀虫剂鱼藤酮对常见害虫斜纹夜蛾表现低毒的原因,研究该药与其他药剂混用的增效机理,采用浸叶法测定了鱼藤酮单剂与混剂对斜纹夜蛾幼虫的生物活性,同时测定处理后虫体内3种解毒代谢酶的活力。结果表明:鱼藤酮对斜纹夜蛾幼虫LC50为1 081.41μg/mL(72h),单剂毒力低,该药对斜纹夜蛾中肠解毒酶羧酸酯酶(CarE)的诱导作用明显,诱导率最高为90.26%,推测斜纹夜蛾幼虫对鱼藤酮的解毒效应与该酶有关。当鱼藤酮与肉桂油、虫酰肼、Bt混配,杀虫活性显著提高,对解毒酶的抑制作用增强。在防治实践中将鱼藤酮与其他作用机制的药剂合理混用,仍能有效防控斜纹夜蛾。The objective of this study was to identify the un derlying mechanism responsible for the low toxicity of the botanical insecticide rotenone against Spodoptera litura. Additionally, the synergistic effects of rotenone mixtures were investigated. For this purpose, the leaf dipping method was used to assess the biological activity of rotenone and rotenone mixtures on S. litura larvae. Meanwhile, the activities of three detoxification metabolic enzymes in S. litura were determined. The results showed that the LC_(50) of rotenone against the third-instar larvae was 1 081.41 μg/mL(72h),indicating a low level of toxicity to S. litura. The carboxylesterase in the midgut of S. litura was significantly affected by rotenone, with a maximum induction rate of 90.26%. It was hypothesized that the detoxification effect of the S. litura larvae to rotenone was related to this enzyme. The synergistic effect of rotenone in combination with cinnamon oil,fenzoylhydrazine, and Bacillus thuringiensis was also studied. The findings revealed that the insecticidal activities of the mixtures were significantly improved, and there was an enhanced inhibition of midgut detoxification enzymes in S. litura.

关 键 词：斜纹夜蛾 鱼藤酮 代谢解毒酶 混剂 增效作用 

分 类 号：S433.4[农业科学—农业昆虫与害虫防治]