复方黄芪通络合剂治疗糖尿病周围神经炎机制  

Discussion on the Mechanism of Compound Astragalus Tongluo Decoction in the Treatment of Diabetic Peripheral Neuritis

作　　者：童雅琴 肖文惠 牟彦竹 张迎庆 TONG Yaqin;XIAO Wenhui;MU Yanzhu;ZHANG Yingqing(School of Biological Engineering and Food,Hubei Univ.of Tech.,Wuhan 430068,China)

机构地区：[1]湖北工业大学生物工程与食品学院,湖北武汉430068

出　　处：《湖北工业大学学报》2024年第5期65-70,共6页Journal of Hubei University of Technology

摘　　要：为探讨复方黄芪通络合剂治疗糖尿病周围神经炎(DPN)的关键有效成分和药理作用机制,通过超高效液相色谱串联质谱(UPLC-MS/MS)进行成分分析,采用网络药理学对其治疗糖尿病周围神经炎的主要信号通路进行预测。实验验证选用60只昆明小鼠进行DPN造模,对小鼠的一般情况、血糖值、热敏值进行记录,对小鼠坐骨神经组织进行HE染色,对PI3K、P-AKT、AKT进行蛋白水平表达的检测。复方黄芪通络合剂经UPLC-MS/MS分析,共鉴定出141个化合物。网络药理学分析显示,70个化合物为有效成分,并推测涉及的生物学过程主要为通过PI3K/AKT信号通路所引起的代谢调节;动物实验验证结果显示,复方黄芪通络合剂虽对小鼠坐骨神经组织中的PI3K的表达水平无明显作用,但给药组中AKT的磷酸化水平显著下降(p<0.05)。复方黄芪通络合剂治疗糖尿病周围神经炎具有多组分、多靶点、多途径的作用机制,为后期该方的深入开发应用奠定了基础。This study aims to investigate the key active components and pharmacological mechanisms of the Compound Astragalus Tongluo decoction in treating diabetic peripheral neuritis.The ingredients of the Compound Astragalus Tongluo decoction were analyzed by the ultra-performance liquid chromatography-high-resolution mass spectrometry(UPLC-MS/MS),its main signaling pathways for the treatment of diabetic peripheral neuritis were predicted by network pharmacology.In experimental verification,60 KM mice were selected for DPN modeling,the general situation,blood glucose value,and heat sensitivity value were recorded,mouse sciatic nerve tissues were HE stained,and protein level expression of PI3K,P-AKT and AKT were detected.There were 141 compounds identified by UPLC-MS/MS analysis,which were divided into 11 categories,and most of them were phenolic acids;Network pharmacology research showed that 70 compounds were active components,and the biological processes involving were mainly metabolic regulated by PI3K/AKT signaling pathway.Animal verification experiments showed that there was no significant effect on the expression level of PI3K in the mouse sciatic nerve tissue;however,the phosphorylation level of AKT was significantly decreased in the administered group(p<0.05).The Compound Astragalus Tongluo decoction for the treatment of diabetic peripheral neuritis via multi-components,multi-targets,and multi-pathways,and laid a foundation for the deeply development and application of this prescription.

关 键 词：复方黄芪通络合剂 糖尿病周围神经炎 网络药理学 

分 类 号：R285[医药卫生—中药学]