平凉地区肉牛腹泻源病毒性腹泻病毒的分离鉴定  

Isolation and Identification of Beef Cattle Diarrhea Virus in Pingliang Region

作　　者：马相雄 丁姝元 常亮[2] 李敏 蒙琦[2] MA Xiangxiong;DING Shuyuan;CHANG Liang;LI Min;MENG Qi(Zhuanglang Animal Husbandry and Veterinary Center,Zhuanglang Gansu 744600,China;Gansu Provincial Institute of Animal Husbandry and Veterinary Medicine,Pingliang Gansu 744000,China)

机构地区：[1]甘肃省庄浪县畜牧兽医中心,甘肃庄浪744600 [2]甘肃省畜牧兽医研究所,甘肃平凉744000

出　　处：《现代畜牧科技》2024年第10期18-20,共3页Modern Animal Husbandry Science & Technology

基　　金：平凉市重点研发计划项目:犊牛病毒性腹泻防治关键技术研究与应用(PL-STK-2023A-037)。

摘　　要：为分离与鉴定平凉地区肉牛腹泻源病毒,采集5份甘肃省平凉地区某大型规模化肉牛养殖场内疑似感染BVDV的肉牛粪便作为研究病料,经MDBK细胞接种分离病毒、病毒含量测定、琼脂扩散试验、RT-PCR、动物回归试验对病毒进行分离与鉴定。结果可见,共分离获得2株BVDV,TCID_(50)值分别为10^(-5.88)/0.1 mL、10^(-5.61)/0.1 mL;这2株病毒经RT-PCR可特异性扩增出1条大小为804 bp的目标电泳条带,与BVDV Oregon C24标准阳性血清的琼脂扩散试验能够产生明显的沉淀线,且与BVDV标准抗原形成的沉淀线相连;接种分离获得的2株病毒至动物体内,可表现出典型的BCVD临床症状。该研究成功从平凉地区肉牛养殖场内分离鉴定出2株BVDV毒株,且毒株的致病性较强,与标准毒株的抗原性相同,可导致肉牛产生典型临床症状。To isolate and identify the source virus of diarrhea in beef cattle in Pingliang area,namely the Habitual Diarrhea Virus.Five suspected BVDV infected beef cattle feces from a large-scale beef cattle breeding farm in Pingliang,Gansu Province were collected as study materials.The virus was isolated and identified by MDBK cell inoculation,virus content determination,agar diffusion test,RT-PCR,and animal regression test.The two strains of BVDV were isolated with TCID_(50) values of 10^(-5.88)/0.1 mL and 10^(-5.61)/0.1 mL,respectively;These two viruses can be specifically amplified by RT-PCR to produce a target electrophoretic band of 804 bp in size.Agar diffusion test with BVDV Oregon C24 standard positive serum can produce a clear precipitation line,which is connected to the precipitation line formed by BVDV standard antigen;Two strains of virus isolated and inoculated into animals can exhibit typical clinical symptoms of BCVD.This study successfully isolated and identified two strains of BVDV from beef cattle farms in Pingliang area,and the pathogenicity of the strains was strong,with the same antigenicity as the standard strain,which can cause typical clinical symptoms in beef cattle.

关 键 词：肉用牛 病毒性腹泻 分离 鉴定 

分 类 号：S858.23[农业科学—临床兽医学]