A microfluidic biosensor for multiplex immunoassay of foodborne pathogens agitated by programmed audio signals  

作　　者：Gaowa Xing Yuting Shang Xiaorui Wang Zengnan Wu Qiang Zhang Jiebing Ai Qiaosheng Pu Ling Lin 

机构地区：[1]Key Laboratory of Ecology-Toxicological Effects and Control for Emerging Contaminants of Fujian Province,College of Environmental&Biological Engineering,Putian University,Putian351100,China [2]Xining Urban Vocational&Technical College,Xining 810000,China [3]Department of Bioengineering,Beijing Technology and Business University,Beijing 100048,China [4]Department of Chemistry,Beijing Key Laboratory of Microanalytical Methods and Instrumentation,Tsinghua University,Bejing 100084,China [5]College of Chemistry and Chemical Engineering,Lanzhou University,Lanzhou 730000,China

出　　处：《Chinese Chemical Letters》2024年第10期370-374,共5页中国化学快报（英文版）

基　　金：supported financially by“Kunlun Talents High-end Innovation and Entrepreneurship Talents”of Qinghai Province in 2022;National Natural Science Foundation of China(Nos.22322401 and 82073816);Beijing Nova Program(No.20220484055)。

摘　　要：Foods are often contaminated by multiple foodborne pathogens,which threatens human health.In this work,we developed a microfluidic biosensor for multiplex immunoassay of foodborne bacteria with agitation driven by programmed audio signals.This agitation,powered by the vibration of a speaker cone during music playing,accelerated the mass transport in the incubation process to form bacterial complexes within 10 min.Immunoassay reagents of the two target bacteria(Escherichia coli O157:H7 and Salmonella typhimurium)were preloaded into the corresponding fore-vacuum storage chamber on the chip,and released to participate in the subsequent immune analysis process by piercing the chambers.All the detection processes were integrated into a single microfluidic chip and controlled by a smartphone through Bluetooth.Under selected conditions,wide linear ranges and low limits of detection(LODs<2CFU/m L)were obtained,and real food samples were successfully determined within 30 min.This biosensing method can be extended to wide-ranging applications by loading different recognizing reagents.

关 键 词：Multiplex immunoassay Microfluidic biosensor Audio signals Foodborne bacteria SMARTPHONE 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置] TS207.4[自动化与计算机技术—控制科学与工程]