A non-peptide-based chymotrypsin-targeted long-wavelength emission fluorescent probe with large Stokes shift and its application in bioimaging  

作　　者：Chuanfeng Fan Jian Gao Yingkai Gao Xintong Yang Gaoning Li Xiaochun Wang Fei Li Jin Zhou Haifeng Yu Yi Huang Jin Chen Yingying Shan Li Chen 

机构地区：[1]Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science,MOE,Shandong Provincial Key Laboratory of Biochemical Engineering,Qingdao Nucleic Acid Rapid Detection Engineering Research Center,College of Marine Science and Biological Engineering,Qingdao University of Science and Technology,Qingdao266042,China [2]School of Chemistry and Life Science,Anshan Normal University,Liaoning Key Laboratory of Development and Utilization for Natural Products Active Molecules,Anshan114007,China [3]Biomedical Analysis Center,Army Medical University,Chongqing 400038,China [4]School of Pharmacy,Weifang Medical University,Weifang 261053,China [5]College of Chemistry,Baicheng Normal University,Baicheng 137000,China

出　　处：《Chinese Chemical Letters》2024年第10期430-436,共7页中国化学快报（英文版）

基　　金：financial support provided by National Natural Science Foundation of China(Nos.21775005,41430641 and 41140032);the Start-Up Fund of Qingdao University of Science and Technology(No.12030430010883)。

摘　　要：As a hydrolase,chymotrypsin(CHT)is involved in many physiological activities,and its abnormal activity is closely related to diabetes,pancreatic fibrosis,chronic pancreatitis and pancreatic cancer.In this work,an innovative long-wavelength emission fluorescent probe TCF-CHT was designed and synthesized for the high specificity detection of CHT,which utilized TCF-OH and a mimetic peptide substrate 4-bromobutyryl as chromogenic group and recognition group,respectively.TCF-CHT exhibited excellent selectivity and eye-catching sensitivity(8.91 ng/m L)towards CHT,“off-on”long-wavelength emission at 670 nm and large Stokes shift(140 nm).Furthermore,the successful fulfillment and perfect performance in imaging endogenous CHT in complex organisms(P815 cells,HepG2 cells,zebrafish and tumor-bearing mice)verified its potential as a powerful tool for the recognition of CHT in complicated biological environments.

关 键 词：CHYMOTRYPSIN Fluorescent probe Long-wavelength emission Large Stokes shift BIOIMAGING 

分 类 号：O657.3[理学—分析化学] Q503[理学—化学]