水飞蓟素衍生物抗肝损伤作用的实验研究  

Experimental Study on Anti-Liver Injury Effects of Silymarin Derivatives

作　　者：张晶 席建军 何若愚 潘金明 庄思静 赵艳梅 曹宇 史婷婷 庄让笑 ZHANG Jing;XI Jianjun;HE Ruoyu;PAN Jinming;ZHUANG Sijing;ZHAO Yanmei;CAO Yu;SHI Tingting;ZHUANG Rangxia(Department of Pharmacy,Xixi Hospital of Hangzhou,Hangzhou,Zhejing 310023)

机构地区：[1]杭州市西溪医院药学部,浙江杭州310023

出　　处：《中国中医药科技》2024年第6期988-991,共4页Chinese Journal of Traditional Medical Science and Technology

基　　金：浙江省基础公益研究计划项目(LGF21H300002);浙江省杭州市科技发展计划项目(20201203B211)。

摘　　要：目的:观察水飞蓟素衍生物抗肝损伤作用并探讨其作用机制。方法:48只小鼠随机分为6组,即正常组,模型组,水飞蓟素衍生物低剂量(25 mg/kg)、中剂量(50 mg/kg)、高剂量(100 mg/kg)组及水飞蓟宾对照组(100 mg/kg),连续灌胃给药5 d。于末次给药1 h后,除正常组外其余各组小鼠腹腔注射给予对乙酰氨基酚(350 mg/kg)进行造模,禁食不禁水,24 h后腹腔静脉取血和肝组织,检测各组小鼠血清学丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)和肝组织丙二醛(MDA)、谷胱甘肽(GSH)以及谷氨酸脱氢酶(GDH)含量,HE染色观察肝组织病理变化。结果:各剂量衍生物能够降低对乙酰氨基酚诱导的肝损伤小鼠血清学ALT、AST指标以及肝组织MDA、GDH水平,并升高GSH含量;同时能够不同程度改善对乙酰氨基酚对肝组织的损伤,展现出不同程度的抗肝损伤活性。结论:水飞蓟素衍生物能够通过调节肝组织MDA、GDH、GSH等指标发挥抗小鼠肝损伤作用。Objective:To evaluate the anti-liver injury effects of Silymarin in vivo.Methods:48 C57BL/6 mice were randomly divided into 6 groups with 8 mice in each group as normal group,model group,23-substituted of Silybin derivative low dose group(25 mg/kg),medium dose group(50 mg/kg),high dose group(100 mg/kg)and Silybin control group(100 mg/kg).They were given relevant drugs(i.g.)every day for 5 d.One hours after the final Ig medicine,APAP(350 mg/kg)was administrated intraperitoneally(i.p.)to each group except normal group.Fasting without water and blood was collected from celiac vein 24 hours la-ter.Serological indexes of ALT,AST,MDA,GSH and GDH of hepatic tissue were determined,HE staining was used to observe the histopathological changes of liver tissue.Results:Different doses of the Silymarin derivatives could decrease the serological ALT and AST indexes,MDA and GDH levels in liver tissue of mice induced by paracetamol,and increase the content of GSH.At the same time,it can improve the liver tissue damage of paracetamol to different degrees,and show different degrees of anti-liver damage activities.Conclusion:Silymarin derivatives can play a certain anti-liver injury effect by regulating MDA,GDH,GSH and other indexes in liver tissue.

关 键 词：水飞蓟宾衍生物 肝损伤 ALT AST MDA GSH GDH 病理学 小鼠 

分 类 号：R285.5[医药卫生—中药学]