不同采收期辛夷挥发油GC-MS分析及其抗氧化、抗菌活性  

GC-MS analysis of volatile oils in Magnoliae Flos at different harvesting periods and their antioxidant and antibacterial activities

作　　者：王杰敏 郭浩川 赵梦薇 孙会改 宋永兴 郑玉光 马东来 WANG Jie-min;GUO Hao-chuan;ZHAO Meng-wei;SUN Hui-gai;SONG Yong-xing;ZHENG Yu-guang;MA Dong-lai(Hebei University of Chinese Medicine,Shijiazhuang 050200,China;Traditional Chinese Medicine Processing Technology Innovation Center of Hebei Province,Shijiazhuang 050091,China;International Joint Research Center on Resource Utilization and Quality Evaluation of Traditional Chinese Medicine of Hebei Province,Shijiazhuang 050091,China;Hebei Chemical and Pharmaceutical Vocational and Technical College,Shijiazhuang 050026,China)

机构地区：[1]河北中医药大学药学院,石家庄050200 [2]河北省中药炮制技术创新中心,石家庄050091 [3]河北省中药资源利用与质量评价国际联合研究中心,石家庄050091 [4]河北化工医药职业技术学院,石家庄050026

出　　处：《药物分析杂志》2024年第9期1475-1484,共10页Chinese Journal of Pharmaceutical Analysis

基　　金：河北省重点研发计划项目(23372504D);河北省中医药管理局科研计划项目(2024094);河北省二期现代农业产业技术体系创新团队项目(HBCT2023080201,HBCT2018060205);道地药材可持续利用项目(202400262157-3)。

摘　　要：目的:分析不同采收期辛夷挥发油的组分和相对含量,探讨5个采收期辛夷化学成分的动态变化规律,并评价其抗氧化、抗菌活性。方法:采用水蒸气蒸馏法对5个采收期辛夷药材进行挥发油提取,利用气相色谱-质谱联用技术分析其化学成分,并计算各成分的相对含量;对5个采收期辛夷的成分进行偏最小二乘法判别分析,筛选出差异性化合物;采用铁离子还原/抗氧化能力法测定辛夷挥发油的抗氧化活性,96孔板法研究其体外抗菌活性。结果:第4采收期(2023年2月10日)辛夷药材总挥发油平均含量最高;从辛夷挥发油中鉴定出38个成分,筛选得到12个差异性化合物,包括γ-衣兰油烯、榄香烯、δ-杜松烯、α-松油醇等,其中γ-衣兰油烯、别香橙烯、2-茨醇、樟脑和顺式-4-侧柏醇在第4采收期相对含量最大,与挥发油含量变化趋势基本一致;5个采收期辛夷挥发油均表现出一定的抗氧化、抗菌活性,第4采收期抗氧化能力最强,且对五种菌的抑制作用均较强。结论:5个采收期辛夷挥发油化学成分基本相同,但在各采收阶段其挥发性成分相对含量有明显差异,推测2月初为辛夷最佳采收期。Objective:To analyze the fractions and relative contents of volatile oils of Magnoliae Flos at different harvesting periods,to elucidate the dynamic pattern of changes in the chemical composition of Magnoliae Flos at five harvesting periods,and to evaluate its antioxidant and antimicrobial activities.Methods:The volatile oils of Magnoliae Flos at five harvesting periods was extracted by water vapour distillation,and the chemical composition was analyzed by gas chromatography-mass spectrometry(GC-MS)technique,and the relative content of each constituent was calculated.The constituents of Magnoliae Flos at the five harvesting periods were analyzed by PLS-DA analysis,which was used in combination with the VIP value to screen out the differential compounds.The antioxidant activity of the volatile oil of Magnoliae Flos was determined by ferric ion reducing antioxidant power(FRAP)method,and its in vitro antimicrobial activity was investigated by 96-well plate method.Results:The total volatile oils content of Magnoliae Flos was the highest in samples at the 4th harvesting period(10 February 2023).Thirty-eight components were identified in the volatile oils of Magnoliae Flos,and 12 differential compounds were screened,includingγ-muurolene,elemene,δ-cadinene andα-terpineol,etc.The relative contents ofγ-muurolene,alloaeromadendrene,borneol,camphor and cis-4-thujanol were the largest in samples at the 4th harvesting period,which was basically in line with the trend of the change of volatile oil content.The volatile oils in samples at five harvesting period showed certain antioxidant and antibacterial activities.And that in samples at the 4th harvesting period showed the strongest antioxidant activity and the inhibition ability against all five species of bacteria.Conclusion:The chemical composition of the volatile oils in Magnoliae Flos was basically the same in in samples at five harvesting periods,but there is a significant difference in the relative content of its volatile components in each harvesting period,and it

关 键 词：辛夷 挥发油 气相色谱-质谱联用(GC-MS) 偏最小二乘法判别分析(PLS-DA) 抗氧化 抗菌 

分 类 号：R917[医药卫生—药物分析学]