利用6-巯基嘌呤的细胞增殖毒性评价转移因子的生物活性  

Assessment of transfer factor bioactivity under 6-mercaptopurine treatment by cell proliferation assay

作　　者：矫建 邓洋 李雅君 牛玉娟 毛希琴 刘进朋 JIAO Jian;DENG Yang;LI Ya-jun;NIU Yu-juan;MAO Xi-qin;LIU Jin-peng(Dalian Center for Certification and Food and Drug Control,Dalian 116021,China)

机构地区：[1]大连市检验检测认证技术服务中心,大连116021

出　　处：《药物分析杂志》2024年第9期1524-1528,共5页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立基于人白血病T细胞株Jurkat E6-1的转移因子(TF)生物活性检测方法。方法:采用细胞毒性和增殖(CCK-8)法探索不同浓度TF对6-巯基嘌呤(6-MP)处理Jurkat E6-1细胞增殖的影响,并对该方法的精密度进行验证。结果:20μg·mL^(-1)TF可使细胞的生存率明显提高,使6-MP的IC50由(0.46±0.10)μg·mL^(-1)右移至(1.11±0.30)μg·mL^(-1);当1.0μg·mL^(-1)6-MP处理细胞时,TF的浓度和细胞生存率具有良好的线性关系,r>0.95。不同批次间在剂量-反应曲线的每个浓度水平上,RSD均小于20%。TF的半数有效浓度(EC50)为(28.49±9.60)mg·mL^(-1),可信限率小于20%。结论:TF可以显著提高暴露于6-MP的Jurkat E6-1细胞生存率,该作用呈剂量依赖性,可为TF生物活性测定提供客观、准确的评价手段。Objective:To develop a method to assess bioactivity of trasfer factor(TF)based on their protective effcets on Jurkat E6-1 cells.Methods:Proliferative effects of different concentrations of TF on 6-mercaptopurine(6-MP)treated Jurkat E6-1 cells detected by CCK-8 assay and the precision was validated.Results:TF exhibited protective activity to change the inhibitory concentration(IC_(50))value of 6-MP from(0.46±0.10)μg·mL^(-1)to(1.11±0.30)μg·mL^(-1)when treated with 20μg·mL^(-1)TF.When cells were treated with 6-MP,there is a good linear relationship between the concentration of TF and cell survival rate,r>0.95.At each concentration level of the dose-response curve,the RSD was less than 20%.The median effective concentration(EC_(50))of TF was(28.49±9.60)μg·mL^(-1),and the confidence limit was less than 20%.Conclusions:TF significantly improved Jurkat E6-1 cell survival rate in dose dependent manner when challenged with 6-MP.It may be suitable for evaluate bioactivity of TF.

关 键 词：转移因子 6-巯基嘌呤 Jurkat E6-1细胞 生物活性 

分 类 号：R917[医药卫生—药物分析学]