滨蒿总黄酮抗呼吸道病毒的体外活性评价  

Anti-respiratory virus effects of the total flavonoids from Artemisia scoparia in vitro

作　　者：马雪萍 史玉柱 王雪 MA Xueping;SHI Yuzhu;WANG Xue(Xinjiang Key Laboratory of Uygur Medicine,Institute of Materia Medica of Xinjiang,Urumqi,Xinjiang,830011 P.R.Chin)

机构地区：[1]新疆维吾尔自治区药物研究所维吾尔药重点实验室,新疆乌鲁木齐830011

出　　处：《华西药学杂志》2024年第5期539-544,共6页West China Journal of Pharmaceutical Sciences

基　　金：自治区创新环境(人才、基地)建设专项-科技创新基地建设(资源共享平台建设)项目(PT2305)。

摘　　要：目的考察滨蒿总黄酮体外抗呼吸道合胞病毒、柯萨奇病毒B3株、甲型流感病毒H1N1的作用。方法采用呼吸道合胞病毒感染Hep2/A549细胞、柯萨奇病毒B3株感染Hep2细胞、H1N1感染A549/THP1细胞模型,细胞病变评分法和Viral ToxGlo法检测滨蒿总黄酮抗呼吸道病毒的作用;ELISA法检测滨蒿总黄酮对病毒感染细胞上清中白介素-1β(IL-1β)、IL-6和肿瘤坏死因子α(TNF-α)的含量。结果滨蒿总黄酮在体外对呼吸道病毒有明显的抑制作用,呈量效关系;滨蒿总黄酮还通过下调IL-6和TNF-α的表达来参与病毒介导的炎症反应。结论滨蒿总黄酮在体外表现出良好的抑制呼吸道病毒作用。OBJECTIVE To investigate the effects of total flavonoids from Artemisia scoparia Waldst.et Kit.(ASTF)on respiratory syncytial virus(RSV),Coxsackie virus B3(CBV3)and influenza A(H1N1)in vitro.METHODS Models(RSV-infected Hep2/A549 cells,CBV3-infected Hep2 cells and H1N1-infected A549/THP1 cells)were used to detect the anti-respiratory virus effect of ASTF in vitro,Cytopathic effect scoring and Viral ToxGlo were used to evaluate the anti-respiratory virus effect of ASTF;ELISA method was further conducted to elucidate the impact of ASTF on the secretion of inflammatory cytokines IL-1β,IL-6 and TNF-αin the supernatant infected by respiratory virus.RESULTS ASTF showed an inhibitory effect on respiratory viruses in vitro.With increasing concentration,the antiviral effect was enhanced in a dose-dependent way.ASTF was also involved in the virus-mediated inflammatory response by decreasing the expression of IL-6 and TNF-α.CONCLUSION ASTF demonstrated good inhibitory effect on respiratory virus in vitro.

关 键 词：滨蒿总黄酮 人肺癌A549细胞 人喉癌Hep2细胞 人急性单核THP-1细胞 炎性细胞因子 呼吸道合胞病毒 柯萨奇病毒B3株 甲型流感病毒H1N1 

分 类 号：R96[医药卫生—药理学]