5种蓟属药用植物的ITS2序列分析  

ITS2 sequence analysis of 5 medicinal plants of Cirsium

作　　者：陈香梅[1,2] 春香[1,2] 青云 乌恩 CHEN Xiangmei;CHUN Xiang;QING Yun;WU En(School of Mongolian Medicine,Inner Mongolia Minzu University,Tongliao,Inner Mongolia,028000 P.R.China;Key Laboratory of Mongolian Medicine Research and Development Engineering,Ministry of Education,Inner Mongolia Minzu University,Tongliao,Inner Mongolia,028000 P.R.China)

机构地区：[1]内蒙古民族大学蒙医药学院,内蒙古通辽028000 [2]内蒙古民族大学蒙医药研发工程教育部重点实验室,内蒙古通辽028000

出　　处：《华西药学杂志》2024年第5期563-566,共4页West China Journal of Pharmaceutical Sciences

基　　金：2020年内蒙古自治区蒙医药标准化项目(2020MB030);蒙药研发工程教育部重点实验室开放基金项目(MDK2024030)。

摘　　要：目的对5种蓟属药用植物58份样品的ITS2序列进行分子鉴定,探讨蓟属药用植物的亲缘关系。方法采用植物基因组DNA提取试剂盒提取58份样本中的DNA,对ITS2序列进行PCR扩增并测序;用MEGA 7.0计算其种间、种内的K2P距离,构建其NJ系统发育树。结果5种蓟属药用植物的ITS2序列长度为227~228 bp,GC含量为59.65%~63.60%;种内最大遗传距离为0.0089,种间遗传距离为0.0179~0.0796;ITS2序列的二级结构中主环、Ⅰ区和Ⅲ区茎环的数目和大小可有效区别;NJ系统发育树分析发现:莲座蓟和绒背蓟的亲缘关系较近。结论ITS2序列可作为5种蓟属药材鉴别的DNA条形码序列。OBJECTIVE To identify the ITS2 sequences of 58 samples from 5 species of medicinal plants of Cirsium by DNA barcode technology,and to discuss the genetic relationship of medicinal plants of Cirsium.METHODS The total DNA was extracted from 58 samples of 5 species of Cirsium using the plant genome DNA extraction kit,and the ITS2 sequence was amplified by PCR and sequenced.The interspecific and intraspecific Kimura 2-parameter distances were calculated with Mega 7.0 to construct its neighbor-joining phylogenetic tree.RESULTS The ITS2 sequence length of five species of Cirsium medicinal plants was 227-228 bp,and the GC%content ranged from 59.65%to 63.60%.The maximum intraspecific genetic distance was 0.0089,and the interspecific genetic distance was 0.0179-0.0796.Through the secondary structure of ITS2 sequence,the number and size of stem rings in regionⅠand regionⅢcan be effectively distinguished.NJ phylogenetic tree analysis showed that the genetic relationship between Cirsium esculentum and Cirsium vlassovianum is close.CONCLUSION ITS2 sequence can be used as DNA barcode sequence for identification of five medicinal materials of Cirsium.

关 键 词：蓟属 莲座蓟 大蓟 小蓟 烟管蓟 绒背蓟 DNA条形码 ITS2序列分析 

分 类 号：R28[医药卫生—中药学]