UPLC法测定不同产地异叶青兰中的9种成分  

Determination of nine chemical components in Dracocephalum heterophyllum from different regions by UPLC

作　　者：曹璐 胡娜[1] 石秘 宋玉 王洪伦[1] CAO Lu;HU Na;SHI Mi;SONG Yu;WANG Honglun(Qinghai Provincial Key Laboratory of Tibetan Medicine Research,Key Laboratory of Tibetan Medicine Research,Northwest Institute of Plateau Biology,Chinese Academy of Sciences,Xining,Qinghai,810008 P.R.China;University of the Chinese Academy of Science,Beijing,100049 P.R.China)

机构地区：[1]中国科学院西北高原生物研究所、中国科学院藏药研究重点实验室、青海省藏药研究重点实验室,青海西宁810008 [2]中国科学院大学,北京100049

出　　处：《华西药学杂志》2024年第5期581-585,共5页West China Journal of Pharmaceutical Sciences

基　　金：青海省创新平台建设专项(2021-ZJ-T05)。

摘　　要：目的采用超高效液相色谱法同时测定异叶青兰中新绿原酸、绿原酸、隐绿原酸、毛蕊花糖苷、木犀草苷、迷迭香酸、香叶木素-7-O-葡萄糖醛酸苷、槲皮素、木犀草素等化学成分的含量。方法采用Zorbox SB-C18色谱柱(100 mm×4.6 mm,1.8μm),流动相为0.1%甲酸甲醇溶液-0.1%甲酸水溶液,梯度洗脱,流速0.3 mL·min^(-1),柱温35℃,检测波长340 nm,进样量2μL。结果9种成分分离较好,线性关系均良好(r^(2)>0.9978),平均加样回收率90.25%~112.61%,RSD均<1.25%。结论所用方法简便、重复性好、结果准确,可为异叶青兰的质量控制提供参考。OBJECTIVE To simultaneously determine nine chemical components in Dracocephalum heterophyllum Benth.,including neochlorogenic acid,chlorogenic acid,cryptochlorogenic acid,acteoside,luteolin-7-O-glucoside,rosmarinic acid,diosmetin 7-O-β-D-glucuronide,quercetin and luteolin by UPLC.METHODS The analysis was performed on a Zorbox SB-C_(18)(100 mm×4.6 mm,1.8μm)column with the mobile phase composed of 0.1%formic acid methanol-0.1%formic acid water solution by gradient elution at a flow rate of 0.3 mL·min^(-1).The wavelength was set at 340 nm with the column temperature of 35℃and the injection volume of 2μL.RESULTS All of the 9 components have been well separated and exhibited good linear relationships(r^(2)>0.9978).The average recoveries of nine compounds were in the range of 90.25%-112.61%,and the RSD values were less than 1.25%.CONCLUSION This method is simple and reliable,with good repeatability and accurate results,which could provide a reference for the quality control of D.heterophyllum.

关 键 词：异叶青兰 超高效液相色谱法 含量测定 绿原酸 槲皮素 毛蕊花糖苷 木犀草苷 迷迭香酸 木犀草素 香叶木素-7-O-葡萄糖醛酸苷 

分 类 号：R917[医药卫生—药物分析学]