MTT法检测积雪草苷脂质体凝胶剂对B16F10小鼠黑色素细胞活性的影响  

Effect of Asiaticoside Liposome Gel on the Activity of B B1616F1010 Cells Detected by MTT Method

作　　者：罗爱勤 钟春燕 钟志勇 黄晓 LUO Aiqin;ZHONG Chunyan;ZHONG Zhiyong;HUANG Xiao(Department of Pharmacy,Guangzhou Xinhua University,Guangdong Province,Guangzhou 510520,China;Zhuhai BesTest Bio-Tech Co.,Ltd,Zhuhai,Guangdong Province,Zhuhai 519040,China)

机构地区：[1]广州新华学院药学院,广东广州510520 [2]珠海百试通生物科技有限公司,广东珠海519040

出　　处：《中国中医药现代远程教育》2024年第22期174-177,共4页Chinese Medicine Modern Distance Education of China

基　　金：广东省教育厅广东省普通高校特色创新类项目【No.2019KTSCX229】;中山大学新华学院校级重点学科项目【No.2020XZD03】;广州新华学院校级教学质量与教学改革工程建设项目【No.2022JYS002】。

摘　　要：目的探讨积雪草苷脂质体凝胶剂对B16F10小鼠黑色素细胞活性的影响。方法体外培养B16F10细胞,以不同浓度积雪草苷、积雪草苷普通凝胶剂、积雪草苷脂质体凝胶剂进行孵育,以四甲基偶氮唑盐法(MTT法)测定各组细胞的活力。结果积雪草苷、积雪草苷普通凝胶剂和积雪草苷脂质体凝胶剂均显示出体外降低B16F10小鼠黑色素细胞活力的作用;积雪草脂质体凝胶剂降低B16F10小鼠黑色素细胞活力的作用明显高于同等药物浓度的积雪草苷普通凝胶剂和相当药物浓度的积雪草苷。结论脂质体凝胶剂剂型有利于药物发挥疗效,积雪草苷脂质体凝胶剂能降低B16F10小鼠黑色素细胞活性,有望应用于治疗皮肤黑色素瘤。Objective To investigate the effect of asiaticoside liposome gel on the activity of melanocytes in mice(B16F10).Methods B16F10 cells were cultured in vitro and incubated with different concentrations of asiaticoside,common gel of asiaticoside,asiatico-side liposome gel.The cell viability of each group was determined by the tetramethylazozolium salt method(MTT method).Results Asiaticoside,common gel of asiaticoside and asiaticoside liposome gel showed the effect of reducing the activity of melanocytes in mice(B16F10)in vitro.The effect of asiaticoside liposome gel on reducing the activity of melanocytes in mice was significantly higher than that of common gel of asiaticoside with the same drug concentration and asiaticoside with the equivalent drug concentration.Con-clusion The dosage form of liposome gel is conducive to the efficacy of drugs,and asiaticoside liposome gel can reduce the activity of melanocytes in mice(B16F10),which is expected to be used in the treatment of skin melanoma.

关 键 词：黑色素瘤 积雪草苷 脂质体凝胶剂 MTT 

分 类 号：R285.5[医药卫生—中药学]