心肌梗死后树突状细胞调节自然杀伤性T细胞相关通路研究  

Dendritic cells regulate natural killer T cell related pathways after myocardial infarction

作　　者：邓宇阳 梁振洋 张大力 何佳奇 DENG Yu-yang;LIANG Zhen-yang;ZHANG Da-li;HE Jia-qi(Department of Cardiology,General Hospital of Northern Theater Command,Shenyang 110016,China)

机构地区：[1]北部战区总医院心血管内科,辽宁沈阳110016

出　　处：《临床军医杂志》2024年第10期1014-1016,1021,共4页Clinical Journal of Medical Officers

基　　金：国家自然科学基金青年项目(82200431)。

摘　　要：目的探讨心肌梗死后树突状细胞调节自然杀伤性T细胞的相关通路。方法利用公共ArrayExpress数据库检索小鼠心肌梗死模型单细胞测序数据,然后通过生物信息学方法在转录水平挖掘树突状细胞在心肌梗死后的调控网络,从而进一步确定其调节自然杀伤性T细胞的通路。结果通过对小鼠模型不同时间点的单细胞测序数据进行聚类分析,共确定了7种免疫细胞:中性粒细胞、单核细胞Ⅰ和Ⅱ、B细胞、巨噬细胞、自然杀伤性T细胞及树突状细胞。细胞间通讯分析及受体-配体分析确定树突状细胞主要通过Selplg-Sell通路调节心肌梗死后自然杀伤性T细胞的浸润。结论树突状细胞在心肌梗死后免疫调节方面发挥重要作用,并通过Selplg-Sell通路调节自然杀伤性T细胞的浸润。Objective To analyze the possible pathways of dendritic cell regulation natural killer T cells after myocardial infarction.Methods Single-cell Selplg-Sell pathway data from a mouse model of myocardial infarction were retrieved using the public ArrayExpress database,and then dendritic cells were analyzed at the transcriptional level for the regulatory network after myocardial infarction by bioinformatics methods,so as to further identify their pathways for regulating natural killer T cells.Results Through clustering analysis of single-cell sequencing data at different time points in the mouse model,a total of 7 immune cells were identified:neutrophils,monocytesⅠandⅡ,B cells,macrophages,natural killer T cells and Dendritic cells.Intercellular communication analysis and ligand pair analysis confirmed that dendritic cells regulate the infiltration of natural killer T cells after myocardial infarction mainly through the Selplg-Sell pathway.Conclusion Dendritic cells play an important role in immunomodulation after myocardial infarction and regulate the infiltration of natural killer T cells through the Selplg-Sell pathway.

关 键 词：心肌梗死 树突状细胞 自然杀伤性T细胞 单细胞测序 Selplg-Sell通路 

分 类 号：R541.4[医药卫生—心血管疾病]