基于正交试验设计的清肝合剂提取工艺优选研究  

Research on Optimization of Extraction Process of Qinggan Mixture Based on Orthogonal Experimental Design

作　　者：王玉 WANG Yu(Department of Pharmacy,Xuzhou Hospital of Traditional Chinese Medicine,Xuzhou Jiangsu 221009,China)

机构地区：[1]徐州市中医院药学部,江苏徐州221009

出　　处：《抗感染药学》2024年第8期780-784,共5页Anti-infection Pharmacy

基　　金：徐州市重点研发计划(社会发展)项目(No.KC22189)。

摘　　要：目的:通过正交试验设计优选清肝合剂的提取工艺,为清肝合剂的生产和质量控制提供参考。方法:采用正交试验设计对清肝合剂提取工艺中的煎煮次数、煎煮时间和加水量进行考察,并以干浸膏得率、黄芩苷转移率和工艺综合成本作为评价指标,优选最佳工艺路线并进行验证。结果:通过正交试验设计发现,煎煮次数为3次、煎煮时间为1 h、加水量为8倍是清肝合剂提取工艺的最优路线,此时干浸膏得率(33.56%)、黄芩苷转移率(80.05%)和工艺综合成本均较为理想,并且工艺的稳定性和重复性也较好。结论:通过正交试验设计优选得到的清肝合剂提取工艺路线具有较好的稳定性和重现性,可用于清肝合剂的生产。Objective:To optimize the extraction process of Qinggan Mixture through orthogonal experimental design,and provide a reference for the production and quality control of Qinggan Mixture.Methods:The orthogonal experimental design was used to investigate the frequency of decoctions,decoction time and water addition in the extraction process of Qinggan Mixture;the dry extract yield,baicalin transfer rate and comprehensive cost of process were used as evaluation indicators to select and validate the optimal process route.Results:The orthogonal experimental design showed that the optimal route for the extraction process of Qinggan Mixture was as follows:3 times of decoctions,1 hour of decoction time,and 8 times of water addition.Under the optimal route,the dry extract yield(33.56%),baicalin transfer rate(80.05%)and comprehensive cost of process were ideal,and the process stability and repeatability were good.Conclusion:The extraction process route of Qinggan Mixture selected by the orthogonal experimental design has good stability and reproducibility,and can be used for the production of Qinggan Mixture.

关 键 词：清肝合剂 提取工艺 正交试验设计 黄芩苷转移率 干浸膏得率 

分 类 号：R94[医药卫生—药剂学]