黑骨藤追风活络胶囊调节mPGES-1改善胶原诱导性关节炎的作用研究  

Study on the Effect of Heiguteng Zhuifeng Huoluo Capsule in Regulating mPGES-1 to Improve Collagen-induced Arthritis

作　　者：罗进芳 何吉 乐岚 何康 黄勇其 姚血明 黄聪 LUO Jinfang;HE Ji;LE Lan;HE Kang;HUANG Yongqi;YAO Xueming;HUANG Cong(Department of Pharmacology,School of Basic Medicine,Guizhou University of Traditional Chinese Medicine,Guiyang 550025,Guizhou,China;Key Laboratory on the Property&Effect of Chinese Medicine(Ethnic Medicine),Guiyang 550025,Guizhou,China;College of Pharmacy,Guizhou University of Traditional Chinese Medicine,Guiyang 550025,Guizhou,China;Department of Rheumatism and Immunology,the Second Affiliated Hospital of Guizhou University of Traditional Chinese Medicine,Guiyang 550025,Guizhou,China)

机构地区：[1]贵州中医药大学基础医学院药理学教研室,贵州贵阳550025 [2]贵州省高等学校中药(民族药)药性与效应研究重点实验室,贵州贵阳550025 [3]贵州中医药大学药学院,贵州贵阳550025 [4]贵州中医药大学第二附属医院风湿免疫科,贵州贵阳550025

出　　处：《医学信息》2024年第20期57-61,共5页Journal of Medical Information

基　　金：贵州省中医药管理局中医药、民族医药科学技术研究课题(编号:QZYY-2021-012);贵州中医药大学国家与省级科技创新人才团队培育项目(编号:贵中医TD合字[2022]004号);贵州省科技计划项目(编号:黔科合基础-ZK[2022]一般477,黔科合基础-ZK[2021]一般537)。

摘　　要：目的研究黑骨藤追风活络胶囊(HZC)调节mPGES-1干预类风湿关节炎的作用机制。方法随机选取24只SD大鼠并分为正常组、模型组、HZC组、甲氨蝶呤(methotrexate,MTX)组,造模成功后对大鼠进行灌胃给药,除正常组外,其余三组采用牛Ⅱ型胶原和完全弗氏佐剂混合乳化剂构建胶原诱导性关节炎(collagen-induced arthritis,CIA)模型,大鼠分别给予生理盐水、MTX和HZC灌胃,共给药21 d。比较各组足肿胀、脾指数、微粒体前列腺素E2合酶-1(microsomal prostaglandin E synthase 1,mPGES-1)的表达情况。结果与正常组大鼠比较,模型组大鼠足肿胀程度明显且伴随着关节变形肿大,HZC组和MTX组大鼠未见明显足肿胀和关节变形。模型组给药第14、21天足肿胀厚度高于正常组,差异有统计学意义(P<0.05),而HZC组和MTX组给药后第14、21天足肿胀厚度低于模型组,差异有统计学意义(P<0.05)。模型组脾指数高于正常组、HZC组、MTX组,差异有统计学意义(P<0.05);与正常组比较,模型组大鼠关节滑膜组织m PGES-1蛋白表达上升(P<0.05),而与模型组比较,HZC组和MTX组mPGES-1蛋白表达下调(P<0.05)。结论HZC能通过干预m PGES-1的表达情况而改善CIA大鼠的炎症反应,起到治疗作用。Objective To study the mechanisms of Heiguteng Zhuifeng Huoluo cupsule(HZC)on collagen-induced arthritis(CIA)rats by regulating mPGES-1.Methods A total of 24 SD rats were randomly selected and divided into normal group,model group,HZC group and methotrexate(MTX)group.After the model was successfully established,the rats were given intragastric administration.Except for normal group,collagen-induced arthritis(CIA)model were constructed with bovine typeⅡcollagen and complete Freund's adjuvant mixed emulsifier.Rats were given normal saline,MTX and HZC by gavage for 21 days.The foot swelling,spleen index and the expression of microsomal prostaglandin E synthase 1(mPGES-1)in each group were compared.Results Compared with the normal group,the degree of foot swelling in the model group was obvious and accompanied by joint deformation and swelling,while there was no obvious foot swelling and joint deformation in the HZC group and MTX group.The thickness of foot swelling in the model group was higher than that in the normal group on the 14th and 21st days after administration,and the difference was statistically significant(P<0.05),while the thickness of foot swelling in the HZC group and MTX group was lower than that in the model group on the 14th and 21st days after administration,and the difference was statistically significant(P<0.05).The spleen index of the model group was higher than that of the normal group,the HZC group and the MTX group,and the difference was statistically significant(P<0.05).Compared with the normal group,the expression of mPGES-1 protein in synovial tissue of rats in the model group increased(P<0.05),while compared with the model group,the expression of mPGES-1 protein in HZC group and MTX group decreased(P<0.05).Conclusion HZC can significantly improve the inflammatory response of CIA rats by interfering with the expression of mPGES-1,and play a therapeutic role.

关 键 词：黑骨藤追风活络胶囊 胶原诱导性关节炎 MPGES-1 

分 类 号：R593[医药卫生—内科学]